敲减FEZF1-AS1通过阻断JAK2/STAT3通路抑制食管鳞状细胞癌细胞的侵袭和迁移

Knockdown of Fez family zinc finger protein 1 antisense ribonucleic acid 1 (FEZF1-AS1) inhibits invasion and migration of esophageal squamous cell carcinoma cells by blocking JAK2/STAT3 pathway

目的探讨长链非编码RNA Fez家族锌指蛋白1反义核糖核酸1(FEZF1-AS1)与食管鳞状细胞癌(ESCC)细胞侵袭、迁移以及与Janus激酶2/信号转导子和转录激活子3(JAK2/STAT3)信号通路的关系。方法用实时定量PCR检测ESCC的癌组织和ESCC细胞系中FEZF1-AS1的表达;构建敲减FEZF1-AS1的慢病毒表达载体,敲减KYSE150、KYSE510细胞的FEZF1-AS1后,流式细胞术检测细胞周期的变化,集落形成实验检测细胞增殖能力,TranswellTM侵袭实验检测细胞侵袭能力,TranswellTM迁移和划痕实验检测细胞迁移能力,Western blot法检测JAK2和磷酸化的STAT3(p-STAT3)蛋白水平。结果在ESCC的癌组织FEZF1-AS1高表达;与正常食管鳞状上皮细胞相比,KYSE150、KYSE510细胞FEZF1-AS1表达高;敲减FEZF1-AS1表达后,ESCC癌细胞的细胞周期和增殖无明显变化,但明显抑制细胞的侵袭和迁移,且JAK2蛋白水平降低,p-STAT3蛋白水平增加。结论敲减FEZF1-AS1阻断JAK2/STAT3通路抑制ESCC细胞的侵袭和迁移。

Objective To investigate the relationship between long chain non-coding RNA Fez family zinc finger protein 1 antisense ribonucleic acid 1(FEZF1-AS1)and invasion,migration and Janus kinase 2/signal transducer and activator of transcription 3(JAK2/STAT3)signal pathway in esophageal squamous cell carcinoma(ESCC)cells.Methods The expression of FEZF1-AS1 in ESCC cancer tissue and ESCC cell line was detected by real-time quantitative PCR.After knocking down the FEZF1-AS1 of KYSE150 and KYSE510 cells by the constructed lentiviral expression vector,the changes of cell cycle were detected by flow cytometry,the ability of cell proliferation by colony formation assay,the ability of cell invasion by TranswellTM invasion assay,the ability of cell migration by TranswellTM migration and scratch assay,and the protein levels of JAK2 and phosphorylated STAT3(p-STAT3)by Western blotting.Results FEZF1-AS1 was highly expressed in ESCC cancer tissues,and FEZF1-AS1 expression in KYSE150 and KYSE510 cells were higher than that in normal esophageal squamous epithelial cells.After the knockdown of FEZF1-AS1 expression,the cell cycle and proliferation of ESCC cancer cells had no obvious change,but cell invasion and migration were significantly inhibited,and the level of JAK2 protein decreased and p-STAT3 protein increased.Conclusion The knockdown of FEZF1-AS1 blocks JAK2/STAT3 pathway and inhibits the invasion and migration of ESCC cells.