2011-2018年广元市HIV-1毒株流行特征分析

Epidemiological characteristics of HIV-1 strain in Guangyuan city

目的了解四川省农业人口较多的广元市1型艾滋病病毒(HIV-1)毒株流行特征。方法获得2011-2018年广元市报告HIV-1感染者的HIV-1毒株pol基因区序列,分析其一般分布特征;并用分子簇分析方法,了解广元市HIV-1流行毒株的成簇特征。结果本研究最终获得广元市833例HIV-1感染者的序列,主要流行毒株为CRF07_BC、CRF01_AE、CRF08_BC和B亚型,占该地区历年检出毒株的94.36%(786例)。2013年以后陆续检出了CRF55_01B、CRF85_BC和CRF67_01B等毒株。广元市HIV-1流行毒株有一定的人群和地域分布特点。其HIV-1毒株总体成簇率为49.94%(416/833)。CRF08_BC和CRF01_AE的成簇率最高,分别为64.10%和56.89%,且不同亚型毒株成簇率差异有统计学意义。发现了129个不同大小分子簇,本地较大(≥10个病例)分子簇簇内病例的主要传播方式有男男同性性行为、异性商业性行为和异性非婚非商业性行为三种。结论近年来,广元市HIV-1毒株类型多样化趋势明显,应加强对HIV-1毒株的特征和流行趋势分析,为后期针对性进行精准干预提供依据。

Objective To understand the epidemiological characteristics of HIV-1 strain in Guangyuan city,an area with a large agricultural population in Sichuan province.Methods Blood samples were collected from 2011 to 2018 and pol gene sequences of HIV-1 strain were detected.The general distribution characteristics of HIV-1 strain were analyzed.The transmission characteristics of HIV-1 strain were analyzed by molecular clustering analysis.Results The main epidemic strains in Guangyuan were CRF01_AE,CRF07_BC,CRF08_BC and B subtypes,accounting for 94.36%(786/833)of the detected strains.In recent years,the strains became obviously diverse.CRF55_01B,CRF85_BC,CRF67_01B,01/BC and BC recombinant strains were found successively.The overall clustering rate of HIV-1 strain was 49.94%(416/833),CRF08_BC(64.10%),and CRF01_AE(56.89%)had the highest clustering rates.A total of 129 molecular clusters were found,and the main ways of forming large molecular clusters were male-to-male sexual behavior,commercial heterosexual and heterosexual non-marriage behavior.Conclusion Various forms of imported cases increase the diversity of virus strains in Guangyuan.In recent years,there are three ways of HIV transmission,via homosexual,commercial heterosexual,and non-marital and non-commercial heterosexual behaviors.