波罗蜜叶绿素缺失突变体嫩茎转录组分析

Transcriptome Analysis of Primary Stem of Chlorophyll Deficient Mutants from Artocarpus heterophyllus

为探究叶绿素合成缺失对波罗蜜嫩茎发育的影响,以波罗蜜叶绿素缺失突变体(chlorophyll deficient mutant,CDM)嫩茎为材料进行转录组分析;经de novo组装后获得295869个Unigenes,使用NR、NT、Swissprot、KEGG、KOG、Pfam和GO数据库进行序列比对,共注释了174291个Unigenes。过滤低丰度基因后,筛选出22988个差异基因。与对照(CK)相比,CDM中上调基因有379个,下调基因有22609个。GO分类结果表明,共有3712个基因获得注释,并将其划分为分子功能(molecular function)、细胞组分(cellular component)和生物学过程(biological process)三大类,共48个功能组;此外,有2080个Unigenes参与到19条KEGG通路上,其中在黄酮类生物合成途径中有30个Unigenes。该研究通过转录组测序,分析叶绿素合成缺失对波罗蜜嫩茎发育的影响,为木本植物茎发育的研究提供数据基础。

To explore the influence of chlorophyll synthesis deficiency on the primary stem development of Artocarpus heterophyllus,the transcriptome of the primary stem of a chlorophyll deficient mutant(CDM)of A.heterophyllus was analyzed.After assembly by de novo,295869 Unigenes were obtained.NR,NT,Swissprot,KEGG,KOG,Pfam and GO databases were used for sequence alignment.A total of 174291 Unigenes were annotated.After filtering the low abundance genes,22988 differentially expressed genes were selected.Compared with the control check(CK),there were 379 genes up-regulated in CDM and 22609 genes down-regulated.GO classification results showed that 3712 genes were annotated and classified into three categories:molecular function,cellular component and biological process,with a total of 48 functional groups.In addition,2080 Unigenes were involved in 19 KEGG pathways,30 of which were involved in flavonoids biosynthesis pathways.Through transcriptome sequencing,this paper analyzed the effect of chlorophyll synthesis deficiency on the primary stem development of A.heterophyllus,which would provide data foundation for the study on the stem development of woody plants.