碳源类型对热带假丝酵母水解产有机酸性能的影响

Effect of Carbon Sources on the Production of Organic Acids Using Candida Tropicalis During Hydrolytic Acidification Process

本文利用高效液相色谱,对热带假丝酵母Candida tropicalis 2.4491利用单一碳源(葡萄糖、甲醇、乙醇、硬脂酸)、复合碳源(葡萄糖+甲醇、葡萄糖+乙醇、葡萄糖+硬脂酸)所产有机酸的种类及含量进行了检测。结果表明:碳源类型对热带假丝酵母代谢产有机酸的种类及含量具有一定影响,所产有机酸均以柠檬酸为主,同时含有少量的乙酸、α-酮戊二酸和苹果酸等。

In this paper,the high performance liquid chromatography(HPLC)was used to detect the types and con⁃tents of the organic acids,which were metabolized by Candida tropicalis using the single carbon sources(glucose,methanol,ethanol,stearic acid)and the complex carbon sources(glucose mixed with methanol,ethanol and stearic ac⁃id).The results showed that the types of carbon sources slightly affected the production of organic acids.The main or⁃ganic acids produced were citric acid in all mediums,with a small amount of acetic acid,α-ketoglutarate and malic acids etc.