23例局灶脑皮质发育不良性癫痫患者GAD-65/67的表达分布

Expression and distribution of GAD-65/67 in 23 patients with epileptogenic forcal cortical dysplasia

目的探索局灶脑皮质发育不良(FCD)性癫痫病灶中GAD-65/67表达分布情况。方法收集广州医科大学附属脑科医院和广州医科大学附属第二医院2017年10月~2019年10月手术中切除的病灶标本,23例FCD癫痫患者手术切除病灶为实验组,7例无癫痫发作正常脑组织为对照组,运用免疫组化染色方法,观察GAD-65/67的表达分布,利用电子显微镜对免疫组化载玻片进行拍照,用IEE 7.0软件进行图像色差分析。结果实验组、对照组均可见GAD-65/67表达分布,对照组脑组织观测到GAD-65/67均匀分布于神经纤维及突触,实验组病理组织学观察到神经元形态异常、排列紊乱,GAD-65/67表达分布相应发生改变。结论FCD由于发生大脑皮层发育异常,GAD-65/67分布异常,可能是导致γ-氨基丁酸的抑制功能减弱的重要原因。

Objective To explore the expression and distribution of GAD-65/67 in patients with forcal cortical dysplasia(FCD)epilepsy.Methods Surgical focus specimen of inpatients were collected from the Affiliated Brain Hospital of Guangzhou Medical University and the Affiliated Second Hospital of Guangzhou Medical University from October 2017 to October 2019.The expression of GAD-65/67 was observed by immunohistochemical staining in 23 patients with FCD epilepsy who underwent surgical resection(the experimental group)and 7 normal brain tissues without epilepsy(the control group).All the stained sections were examined under a microscope and photographed with a digital camera and the image chromatic aberration was analyzed by IEE 7.0 software.Results The expression of GAD-65/67 was found in both the experimental group and the control group.In the control group,GAD-65/67 was found to be evenly distributed in nerve fibers and synapses in brain tissue.In the experimental group,abnormal morphology and arrangement of neurons were observed and the expression and distribution of GAD-65/67 were changed correspondingly.Conclusion This study suggests that the abnormal distribution of GAD-65/67 may be attributed to the abnormal development of cerebral cortex in FCD,resulting in the decrease of GABA inhibition.