Radil在肝癌组织中的表达及对肝癌发展的影响

Expression of Ras association and DIL domains(Radil)and its effect on hepatocellular carcinoma

目的:探讨肝癌组织中Radil(Ras association and DIL domains)的表达水平,并进一步分析其对肝癌发展的影响。方法:采用免疫组化和Western blot对手术切除的癌组织及对应的癌旁组织进行蛋白质水平检测;用Western blot方法分析各肝癌细胞系中Radil的表达,并利用敲低或过表达的方法分析Radil表达水平的变化对肝癌细胞生长、运动的影响。结果:Radil在肝癌组织中表达均显著高于对应癌旁组织,高表达Radil可以促进肝癌细胞生长、迁移,而抑制凋亡相关分子的表达。结论:Radil在肝癌组织中高表达,可显著促进肝癌细胞的生长、迁移,有望为肝癌的治疗和预后判断提供参考。

Objective:To assess the expression level of Ras association and DIL domains(Radil),and its effect on hepatocellular carcinoma(HCC).Methods:The expression of Radil was measured by immunohistochemistry and Western blot in HCC tissue samples.Then the expression of Radil was measured in several HCC cell lines by Western blot,and Rnock-down and over-expression were carried out in HCC cell lines respectively to test cell proliferation and movement ability.Results:The expression of Radil in HCC tissues was significantly higher than that in corresponding adjacent tissues.The expression of Radil promoted HCC cell proliferation and migration ability,and the apoptosis-related protein expression levels were decreased.Conclusions:This study suggests that Radil is overexpressed in HCC.It may play an important role in carcinogenesis or aggression and may be a useful marker for the prognosis evaluation of HCC.