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新型冠状病毒高效价中和血清的快速制备及应用 被引量:6

Rapid preparation and application of high titer neutralizing antiserum against SARS-Co V-2
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摘要 目的制备新型冠状病毒高效价中和血清。方法使用重组表达的新型冠状病毒RBD蛋白作为免疫原,免疫SPF级家兔,制备抗血清。通过ELISA和微量中和试验检测抗血清效价。将制备的抗血清分发至我国多家新冠灭活疫苗研发企业,检测抗血清效价。结果经3次免疫后,抗血清的ELISA效价大于160万,中和效价为8192。5家企业返回微量中和效价检测结果的几何平均效价为6950。结论成功制备了新型冠状病毒高效价中和血清,并分发至我国多家新型冠状病毒灭活疫苗生产企业用于毒株鉴别及外源病毒因子检测,解决了制约疫苗快速研发的技术瓶颈。 Objective To prepare high titer neutralizing antiserum against SARS-CoV-2.Methods SPF rabbits were immunized with RBD protein of SARS-CoV-2,expressed in 293 cells,as an immunogen.The prepared antiserum was determined for titer by ELISA and microneutralization test,and distributed to several manufacturers for development of inactivated SARS-CoV-2 vaccine for determination of titer.Results After 3 times of immunization,the antiserum titer reached more than 1600000,while the neutralizing titer was 8192.The geometric mean titer(GMT)determined by microneutralization test in five manufacturers was 6.950.Conclusion High titer neutralizing antiserum against SARSCoV-2 was successfully prepared and distributed to several manufacturers of inactivated SARS-CoV-2 vaccine candidate for virus identification and adventitious virus agent test,which broke through the technical bottleneck restricting the rapid development of SARS-CoV-2 vaccine.
作者 徐康维 王剑锋 权娅茹 邵铭 赵慧 李长贵 王军志 XU Kang-wei;WANG Jian-feng;QUAN Ya-ru;SHAO Ming;ZHAO Hui;LI Chang-gui;WANG Jun-zhi(Division of Respiratory Virus Vaccines,National Institutes for Food and Drug Control,Beijing 102629,China)
出处 《中国生物制品学杂志》 CAS CSCD 2020年第8期918-920,共3页 Chinese Journal of Biologicals
基金 国家重大新药创制(2018ZX09737003-002,2018ZX09738005) 北京市自然科学基金(L192009)。
关键词 新型冠状病毒 抗血清 毒株鉴别 外源病毒因子 SARS-CoV-2 Antiserum Virus identification Adventitious virus agent
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