病毒类生物制品生产工艺的研究进展

Progress in research on production procedure of virus-based biologicals

病毒是由蛋白质外壳和内部的遗传物质(DNA或RNA)组成,利用宿主的细胞系统进行自我复制,无法独立生长和复制。病毒可感染几乎所有具有细胞结构的生命体,可引发多种疾病。目前,对大多数病毒感染引起的疾病均无特效药,接种疫苗是预防这些疾病的有效途径,分离和纯化病毒蛋白是病毒类生物制品生产的主要工艺步骤。本实验从表达体系、工艺开发及检测技术3个方面对病毒类生物制品的研究进展及已成功应用的病毒类生物制品的工艺流程作一综述。

Viruses consist of protein envelope and internal genetic material(DNA or RNA),which are replicated themselves by using host cell system,without the capacity of growing and replicating independently.Viruses may infect almost all the organisms with cell structure,resulting in various diseases.At present,there are no specially effective drugs for most of diseases caused by virus infection,so vaccination is an effective measure to prevent the diseases.Isolation and purification of viral protein are the key technological steps of production of virus-based biologicals.This paper reviews the progress in research on virus-based biologicals and the production procedure of the biologicals applied successfully in the aspects of expression system,procedure development and test technique.