油茶象甲对油茶果实挥发物的触角电生理和行为反应

Electroantennogram and Behavioral Responses of Curculio chinensis to Volatiles of Camellia oleifera Fruits

寄主植物挥发性化合物往往对昆虫搜寻定位寄主及食物选择具有重要的影响,为了明确油茶(Camellia oleifera)果实中是否含有引诱油茶象甲(Curculio chinensis)取食的特定挥发性化合物,研究利用GC-EAD测试了油茶果实挥发物对油茶象甲的触角电位反应,通过GC-MS数据库对活性挥发物进行鉴定,并采用Y型嗅觉仪测试了油茶象甲对活性化合物的行为反应。研究结果表明在15.05、19.30、21.97和22.79 min 4个出峰时间点的物质能够引起油茶象甲较强的触角反应,经过质谱鉴定和标准品比对,确定在15.05和19.30 min出峰的物质分别为正十八烷和油酸酰胺。Y型嗅觉仪选择试验结果表明0.1和1 ng/μL浓度的正十八烷对油茶象甲具有较强的驱避作用,1 ng/μL的油酸酰胺对油茶象甲具有较强的引诱作用。研究确定了正十八烷和油酸酰胺对油茶象甲的行为具有调控作用。

The volatile compounds of host plants could influence the host plants location and food searching of insects.In order to find out whether there is any volatile compound of Camellia oleifera fruits that attract Curculio chinensis,in this study,GC-EAD was used to test the electroantennogram response of C.chinensis to the volatile compounds of C.oleifera fruits,GC-MS was used to identify the active volatile compounds,and Y-olfactometer was used to test the behavioral responses of C.chinensis to purchased pure compound.The results showed that at the retention time of 15.05,19.30,21.97 and 22.79 min,these four compounds could cause strong antennae reaction of C.chinensis.After identifying by mass spectrometry comparing with standard compounds,the peaks at retention time of 15.05 and 19.30 min were determined as n-octadecane and oleamide,repectively.The results of Y-olfactometer showed that 0.1 ng/μL and 1 ng/μL of n-octadecane had strong repellent effect against C.chinensis,and 1 ng/μL of oleamide showed significantly attractive effect on C.chinensis.In this study,we showed that n-octadecane and oleamide had a regulatory effect on the behavior of C.chinensis.