朱顶红鳞茎组织培养中的抗褐化研究

Studies on Anti-browning in Bulbs Tissue Culture of Hippeastrum rutilum

试验以朱顶红(Hippeastrum rutilum)无菌苗为材料,通过筛选基本培养基、低pH水培、添加不同种类和浓度的凝固剂、不同浓度蔗糖、不同褐化剂来探讨鳞茎组织培养中抑制褐化的有效方法。结果表明:朱顶兰在MS基本培养基中生长状况较好,褐化程度最低;低pH水培显著影响朱顶红的生长与增殖,且不能有效抑制朱顶红鳞茎褐化;以琼脂粉A为凝固剂,半固体状态,附加30 g·L^(-1)蔗糖的MS培养基有利于朱顶红鳞茎的抗褐化和增殖;在继代培养基中添加1.0 g·L^(-1)活性炭可有效抗褐化。因此,朱顶红鳞茎继代过程中适宜的培养条件为:MS+2.0 mg·L^(-1) 6-BA +30 g·L^(-1)蔗糖+1.8 g·L^(-1)琼脂粉A或MS+2.0 mg·L^(-1) 6-BA +30 g·L^(-1)蔗糖+6.0 g·L^(-1)卡拉胶+1.0 g·L^(-1)活性炭。

The effective approaches of anti-browning in bulbs tissue culture was studied with the aseptic seedling of Hippeastrum rutilum by selecting basic medium,low pH ydroponic culture,adding different type and concentration of coagulant,different concentration of sucrose,different anti-browning agent.MS medium showed better growth status and lower rate of browning.Low pH hydroponic culture significantly affected the growth status,and could not effectively inhibit the browning of bulb.MS medium with agar powder A(semi-solid state)and sucrose with concentration of 30 g·L^-1 was beneficial to the browning resistance and proliferation.Adding 1.0 g·L^-1 activated carbon to the medium can effectively prevent browning.Therefore,the suitable tissue culture conditions for bulbs of H.rutilum were MS+2.0 mg·L^-16-BA+30 g·L^-1 sucrose+1.8 g·L^-1 agar powder A or MS+2.0 mg·L^-16-BA+30 g·L^-1 sucrose+6.0 g·L^-1 carrageenan+1.0 g·L^-1 activated carbon.