红芪多糖1联合吉非替尼对肺癌A549细胞凋亡的作用及机制探讨

Effect of Hedysarum polysaccharides 1 combined Gefitinib on apoptosis of lung cancer A549 cells and its mechanism research

目的:探讨红芪多糖1(HPS1)联合吉非替尼对肺腺癌A549细胞凋亡的作用机制。方法:MTT法检测不同浓度的HPS1、吉非替尼及二者联用对肺腺癌A549细胞增殖的影响。流式细胞术检测细胞凋亡率及细胞周期分布。实时荧光定量PCR法检测Bcl-2和Bax mRNA的表达情况。结果:各浓度组(50、100、200、400 mg/L)HPS1对A549细胞增殖均有抑制作用,抑制率与浓度和时间呈正相关。HPS1、吉非替尼均可致A549细胞凋亡率增高,且两药联用组较单用吉非替尼组凋亡率明显增加(t=2.412,P<0.05)。各浓度组HPS1均可降低Bcl-2蛋白表达(P值均<0.05),增加Bax蛋白表达(P值均<0.05),具有浓度依赖性;且两药联合处理对Bcl-2和Bax蛋白表达的抑制或促进作用均较单用吉非替尼组增强,差异均有统计学意义(P值均<0.05)。结论:HPS1联合吉非替尼在抑制A549细胞增殖、促使其凋亡方面有协同作用;其机制可能与降低Bcl-2/Bax mRNA比例相关。

Objective To investigate the effect of Hedysarum polysaccharides 1(HPS1)on apoptosis of lung cancer A 549 cells induced by Gefitinib,and to explore its underlying mechanism.Methods The inhibitory effects of different concentrations of HPS1 alone and Gefitinib alone and in combination on the growth of A 549 cells were tested by MTT method.The apoptosis and cell cycle were detected by flow cytometry.The expressions of Bcl-2 and Bax mRNA were measured by real time fluore.scence quantitative-PCR.Results Different concentrations of HPS1(50,100,200,400 mg/L)had inhibitory effects on the growth of A 549 cells in a concentration-and time-dependent manner.The apoptotic rate was significantly increased in A 549 cells treated with HPS1 in combination with Gefitinib,compared with that in Gefitinib-treated A 549 cells(t=2.412,P<0.05).The exprevssion level of Bcl-2 protein was decreased(all P<0.05)and the expression level of Bax was increased(all P<0.05)in a concentration-dependent manner in A 549 cells treated with different concentrations of H PS1,and these effects were significantly enhanced in A 549 cells treated with HPS1 in combination with Gefitinib as compared with those in the cells treated with Gefitinib alone(all P<0.05).Conclusions HPS1 can enhance the apoptotic level of A 549 cells induced by Gefitinib,and this effect may be associated with the decrease in the ratio of Bcl-2/Bax mRNA.