LC3-Ⅱ和ATF3在肝细胞癌中的表达及其相关性研究

Expression and correlation of LC3-Ⅱand ATF3 in hepatocellular carcinoma

目的分析微管相关蛋白1轻链3-Ⅱ(LC3-Ⅱ)和激活转录因子3(ATF3)在肝细胞癌中的表达情况,探讨其蛋白表达与HCC患者预后的关系,并分析在HCC组织中,两者的表达是否具有相关性。方法应用免疫组织化学法检测HCC组织及相应癌旁组织蜡块标本中LC3-Ⅱ与ATF3蛋白的表达情况,并分析其与患者临床病理特征和生存期之间的关系。使用免疫蛋白印迹(Western blot)法检测新鲜HCC组织及相应癌旁组织中LC3-Ⅱ与ATF3蛋白的表达,分析两种蛋白表达的相关性。结果LC3-Ⅱ和ATF3蛋白在癌旁组织中的表达明显高于HCC组织,两者的表达水平与HCC组织病理分级和静脉癌栓情况相关(均P<0.05),但与年龄、性别、血清甲胎蛋白、肿瘤直径、HBsAg等的相关性无统计学意义(均P>0.05)。LC3-Ⅱ和ATF3的低表达与HCC患者的不良预后显著相关(均P<0.05)。结论LC3-Ⅱ和ATF3蛋白表达均与HCC的发生发展有关,两者的联合检测有助于HCC的恶性程度的评估,有望成为判断患者预后的重要指标。

Objective To analyze the expression of LC3-Ⅱand ATF3 in hepatocellular carcinoma(HCC),to explore the relationship between their expression and the prognosis of patients with HCC,and to analyze the expression correlation betweenLC3-Ⅱand ATF3 in HCC tissues.Methods Immunohistochemical method was used to detect the expression of LC3-Ⅱand ATF3 protein in HCC tissue and corresponding paracancerous tissue specimens,to analyze the relationship between these two proteins and the patient's clinicopathological characteristics as well as survival time.Western blot was used to detect the expression of LC3-Ⅱand ATF3 proteins in fresh HCC tissues and corresponding paracancerous tissue.Results The expression of LC3-Ⅱand ATF3 inparacancerous tissues was significantly higher than that in HCC tissues.The expression levels of were related to HCC histopathological grade and venous tumor thrombus(all P<0.05),but were not related to age,gender,and serum alpha-fetoprotein,tumor diameter,HBsAg,etc(all P>0.05).The low expression of LC3-Ⅱand ATF3 was significantly related to the poor prognosis of patients with HCC(both P<0.05).Conclusions Both LC3-Ⅱand ATF3 protein expression are related to the occurrence and development of HCC.The combined detection of LC3-Ⅱand ATF3 is helpful for the assessment of the malignant degree of HCC and it is expected to become an important indicator for judging the prognosis of patients.