预处理及培养方法协同作用对新铁炮百合种子萌发的影响

Synergistic Effect of Pretreatment and Cultural Method on Lilium×fomolongi Germination

【目的】研究不同预处理方式下培养方法协同作用对新铁炮百合种子萌发的影响。【方法】以新铁炮百合雷山1号种子为材料,采用三因素四水平正交试验设计,研究不同水平的预处理方式、培养温度、培养方式对新铁炮百合雷山1号种子萌发的影响,以及不同温度下种子萌发过程中可溶性糖和过氧化物歧化酶(SOD)的动态变化规律。【结果】培养温度是影响新铁炮百合雷山1号种子萌发的主要因素,其次是预处理方式和培养方式。雷山1号种子的最佳预处理方式是4℃浸种7 d,最佳培养温度是21℃,最佳培养方式是土壤播种,发芽率为99.18%,播种后第9 d发芽指数为4.65。不同温度处理下,种子萌发过程中超氧化物歧化酶(SOD)活性表现出先增加后下降的趋势,可溶性糖含量则呈现出先下降再上升的趋势,其中21℃处理下种子的抗逆性和贮藏物质的转化利用要高于15、18和25℃处理下的种子,21℃为适宜温度。【结论】雷山1号种子的最佳处理方式为4℃浸种7 d于21℃下土壤播种。

【Objective】Lilium formolongi Raizan No.1 was used as experimental material,the orthogonal experiment design of three factors and four levels was used to research the effects of different seed pretreatments and cultural temperatures,and cultural method on Lilium formolongi Raizan No.1 seed germination.This experiment also researched the dynamic change of soluble sugars and superoxide dismutase(SOD)in different temperatures.【Results】The results showed that the key factor was cultural temperature,followed by pretreatment and cultural method.And as for Raizan No.1,the best way of pretreatment was soaking seed in 4℃for 7 days,the optimum temperature was 21℃,the optimum culture mode was sowing in soil,its germination was 99.18%and the germination index was 4.65.At different temperatures,the activity of superoxide dismutase(sod)increased first and then decreased at germination stage,but the soluble sugar content showed a tendency of going down first and then up.Among them,the resistance of the seeds under 21℃treatment and the conversion utilization of the stored substances were higher than those under 15℃,18℃and 25℃treatment,which confirmed that 21℃was the appropriate temperature.【Conclusion】The optimum treatment of the Rayshan 1seeds was to sow at 21℃for 4℃for 7 days.