梅毒血清固定患者外周血趋化因子受体、IL-12、IL-10水平变化及意义

Changes and Significance of Chemokine Receptor and IL-12,IL-10 in Peripheral Blood of Patients with Syphilis Serum Fixation

目的:探究梅毒血清固定患者外周血趋化因子受体、IL-12、IL-10水平变化及意义。方法:选取2016年8月-2019年9月本院收治的梅毒血清固定患者38例为固定组,梅毒治疗后非梅毒螺旋体抗原血清试验转阴患者27例为转阴组,以及进行体检健康者33例为健康对照组。采用流式细胞仪双标法检测三组外周趋化因子受体CCR3、CCR5、CXCR4、CXCR3水平,采用双抗体及夹心ELISA法检测三组IL-12、IL-10水平,观察分析三组外周血趋化因子受体、IL-12、IL-10水平的变化及意义。结果:固定组外周血CCR3表达明显高于转阴组与健康对照组(F=255.441,P<0.05),而CCR5、CXCR3表达均低于转阴组与健康对照组(F=70.674、218.128,P<0.05);转阴组与健康对照组外周血细胞CCR3、CCR5、CXCR4、CXCR3比较,差异均无统计学意义(P>0.05);固定组IL-10水平明显高于转阴组与健康对照组(F=48.205,P<0.05),IL-12比转阴组与健康对照组低,但差异无统计学意义(F=1.173,P>0.05);ROC曲线显示,外周血趋化因子受体、IL-12、IL-10水平对梅毒血清固定的AUC依次为0.838、0.797、0.592、0.908、0.503、0.660。结论:外周血趋化因子受体、IL-12、IL-10水平对梅毒血清固定有着一定的诊断价值,对梅毒血清固定患者的病情发展起着很重要的作用。

Objective:To investigate the changes and significance of chemokine receptor and IL-12,IL-10 in peripheral blood of patients with syphilis serum fixation.Method:From August 2016 to September 2019,38 patients with syphilis serum fixation treated in our hospital were selected as the fixed group,after syphilis treatment 27 patients with negative serum test of non-Treponema pallidum antigen were selected as the negative group,and 33 healthy people who underwent physical examination were taken as the healthy control group.The levels of CCR3,CCR5,CXCR4 and CXCR3 of three groups were measured by flow cytometry,and the levels of IL-12,IL-10 of three groups were detected by double antibody and sandwich ELISA.The changes and significance of chemokine receptor and IL-12,IL-10 levels in peripheral blood of three groups were observed and analyzed.Result:The expression of CCR3 in peripheral blood of the fixed group was significantly higher than those of the negative group and the healthy control group(F=255.441,P<0.05),while the expression of CCR5,CXCR3 were lower than those of the negative group and the healthy control group(F=70.674,218.128,P<0.05).The difference of CCR3,CCR5,CXCR4 and CXCR3 in peripheral blood cells between the negative group and the healthy control group were not statistically significant(P>0.05).The level of serum IL-10 in the fixed group was significantly higher than those in the negative group and the healthy control group(F=48.205,P<0.05),but the serum IL-12 in the fixed group was lower than those in the negative group and the healthy control group(F=1.173,P>0.05).The ROC curve showed that the level of the peripheral blood chemokine receptor,IL-12 and IL-10 in the serum of syphilis were 0.838,0.797,0.592,0.908,0.503,0.660.Conclusion:The level of chemokine receptor,IL-12 and IL-10 in peripheral blood has a certain diagnostic value for syphilis serum fixation,and plays an important role in the development of syphilis serum fixation patients.