姜黄素通过NF-κB信号通路发挥对S16细胞的保护作用

Protection Effect of Curcumin on S16 Cells through NF-κB Signaling Pathway

目的:探讨姜黄素对受阿霉素神经毒性损害的S16细胞(大鼠施万细胞)的保护作用,并分析其作用的分子机制。方法:以正常培养的S16细胞培养基作为对照组,以400 ng/mL阿霉素刺激S16细胞培养基作为阿霉素组,阿霉素组中含100μg/mL姜黄素的培养基作为姜黄素组。采用噻唑蓝(MTT)实验与Western Blot测定并比较三组S16细胞增殖情况与Caspase-8凋亡蛋白相对含量;利用酶联免疫吸附实验(ELISA)测定并比较三组NF-κB、肿瘤坏死因子-α(TNF-α)与超氧化物歧化酶(SOD)表达量。结果:阿霉素组OD值为(0.82±0.21),比对照组的(1.84±0.16)降低(t=13.33,P<0.01);姜黄素组OD值为(1.19±0.14),比阿霉素组升高(t=5.08,P<0.01)。阿霉素组Caspase-8凋亡蛋白相对含量为(37.14±0.02),比对照组的(8.93±0.04)升高(t=1524.54,P<0.01);姜黄素组Caspase-8凋亡蛋白相对含量为(24.62±0.07),比阿霉素组降低(t=504.17,P<0.01),姜黄素组NF-κB与SOD含量均高于阿霉素组(t=184.54、52.36,P<0.01),姜黄素组TNF-α含量低于阿霉素组(t=64.49,P<0.01)。结论:姜黄素通过NF-κB信号通路保护受阿霉素神经毒性损害的S16细胞。姜黄素与阿霉素联合应用可以降低阿霉素产生的神经毒性副作用,是比较有前途的肿瘤化疗抗副作用药物。

Objective:To investigate the protective effect of Curcumin on S16 cells(Schwann cells in rats)damaged by Adriamycin neurotoxicity,and to analyze its molecular mechanism.Method:The normal S16 cell culture medium was used as the control group,400 ng/mL Adriamycin stimulated S16 cell culture medium was used as the Adriamycin group,and the 100μg/mL Curcumin medium in the Adriamycin group was used as the Curcumin group.The proliferation of S16 cells and the relative content of caspase-8 apoptosis protein among three groups were measured and compared by Thiazoline-blue(MTT)experiment and Western blot.The expression of NF-κB,tumor necrosis factor-α(TNF-α)and superoxide dismutase(SOD)among three groups were measured and compared by enzyme-linked immunosorbent assay(ELISA).Result:The OD value of the Adriamycin group was(0.82±0.21),it was lower than(1.84±0.16)of the control group(t=13.33,P<0.01).The OD value of the Curcumin group was(1.19±0.14),it was higher than that of the Adriamycin group(t=5.08,P<0.01).The relative content of the Caspase-8 apoptosis protein in the Adriamycin group was(37.14±0.02),it was higher than(8.93±0.04)in the control group(t=1524.54,P<0.01).The relative content of Caspase-8 apoptosis protein in the Curcumin group was(24.62±0.07),it was lower than that in the Adriamycin group(t=504.17,P<0.01).The content of NF-κB and SOD in the Curcumin group were higher than those in the Adriamycin group(t=184.54,52.36,P<0.01).The content of TNF-αin the Curcumin group was lower than that in the Adriamycin group(t=64.49,P<0.01).Conclusion:Curcumin protects S16 cell damaged by Adriamycin neurotoxicity through the NF-κB signaling pathway.Curcumin combined with Doxorubicin can reduce the neurotoxic side effects of Doxorubicin and is a promising anti-side effect drug for tumor chemotherapy.