氮受控对酒糟废水-微藻培育耦合体系的影响

Effect of nitrogen control on the coupling system of brewery waste water and microalgae cultivation

为考察酒糟废水-微藻培育耦合体系的最适氮营养条件,运用室内受控实验,调控酒糟废水总氮浓度,考察莱茵衣藻、二形栅藻分别在单一培养和共培养条件下的生长特性,计算酒糟废水中营养盐的去除率.研究结果显示,较之二形栅藻,莱茵衣藻对氮的需求更为敏感.当初始氮磷质量比为2.45,且初始总氮(total nitrogen,TN)质量浓度为40.25 mg/L时,对莱茵衣藻,最终生物量为879.50 mg/L,藻蛋白质量浓度达69.00 mg/L,对总氮、总磷(total phosphorus,TP)和化学需氧量(chemical oxygen demand,COD)的去除率分别为93.68%、90.81%和72.57%;对二形栅藻,最终生物量为775.00 mg/L,藻蛋白质量浓度为154.53 mg/L,对TN、TP和COD的去除率分别为96.85%、70.80%和82.06%.共培养条件下,莱茵衣藻在生长和对氮磷等资源的利用方面受二形栅藻的抑制影响较明显,当初始氮磷质量比为2.45,初始TN质量浓度为40.25 mg/L时,莱茵衣藻对TN和TP的去除率分别为97.37%%和74.74%;共培养条件下微藻对酒糟废水COD的去除结果与二形栅藻类似.研究发现,利用酒糟废水-微藻培育耦合体系、单一培养体系和共培养体系,酒糟废水水质均能满足地表水环境质量标准的IV类水总氮(≤1.5 mg/L)和发酵酒精与白酒工业水污染物排放标准的要求.

In order to investigate the optimal nitrogen nutrition conditions of the brewery waste water-microalgae cultivation coupling system,the control variable method was used to regulate the total nitrogen concentration of the brewery waste water.The growth characteristics of Chlamydoomonas reinhardtii and Scenedesmus dimorphus under single culture and co-culture conditions were observed,and the absorption and removal efficiency of nutrients in brewery waste water was explored.The results show that the nitrogen requirement of Chlamydoomonas reinhardtii was higher than that of Scenedesmus dimorphus.When the initial total nitrogen(TN)concentration was 40.25 mg/L and the initial nitrogen to phosphorus ratio was 2.45,for Chlamydoomonas reinhardtii,the final biomass reached 879.50 mg/L,algae protein content reached 69.00 mg/L,and total nitrogen,total phosphorus(TP),organic matter(chemical oxygen demand,COD)removal rate reached 93.68%,90.81%and 72.57%,respectively.For Scenedesmus dimorphus,the biomass reached 775 mg/L,and the content of algae protein reached 154.53 mg/L.The final removal rates of total nitrogen,TP,COD reached 96.85%,70.80%and 82.06%,respectively.Under the co-culture condition,the growth and utilization of nitrogen and phosphorus in Chlamydoomonas reinhardtii were obviously affected by the inhibition of Scenedesmus dimorphus.When the initial TN concentration was 40.25 mg/L,the initial nitrogen-phosphorus ratio was 2.45,the removal rates of TN and TP were 97.37%%and 74.74%.However,the removal of COD from brewery waste water by microalgae under co-culture conditions was similar to that of Scenedesmus dimorphus.The study show that through the brewery waste water-microalgae cultivation coupling system,whether it is a single or co-culture system,the brewery waste water can meet the total nitrogen requirement(1.5 mg/L)of class IV water in the surface water environmental quality standard.