结直肠癌组织中长链非编码RNA LINC01617的表达及其对结直肠癌细胞增殖的作用

Expression of long noncoding RNA LINC01617 in colorectal cancer tissues and its effect on proliferation of colorectal cancer cells

目的探讨长链非编码RNA LINC01617在结直肠癌组织与对应癌旁组织中的差异表达,分析其表达水平与结直肠癌临床病理学特征,预后生存期和肿瘤细胞恶性表型的关系。方法使用实时荧光定量PCR法检测2007年3月至20011年12月于曙光医院肿瘤科接受治疗的152例结直肠癌患者的结直肠癌组织与对应癌旁组织中LINC01617表达量,分析结直肠癌组织中LINC01617表达水平与临床病理学特征和预后生存期的关系。在结直肠癌细胞系中感染LINC01617干扰病毒以模拟功能缺失实验,以MTT实验检测细胞增殖能力。结果结直肠癌组织中lncRNA LINC01617表达水平显著高于癌旁组织(8.2±7.42比2.32±1.34,P<0.001),LINC01617在结直肠癌组织中的表达水平与年龄、性别无相关性(均P>0.05),但与肿瘤大小,淋巴结转移和病理临床分期显著相关(P<0.001,P<0.001,P=0.003)。LINC01617表达量与结直肠癌患者预后生存期有显著的负相关性(P<0.01)。干扰LINC01617表达可诱导结直肠癌细胞系HCT116和Lovo细胞增殖能力显著抑制,差异均有统计学意义(均P<0.05)。结论lncRNA LINC01617在结直肠癌患者组织中表达量异常升高,且其异常高表达和患者的恶性临床表征和更短的预后生存期相关。LINC01617对结直肠癌细胞的生长有促进作用,表明LINC01617的异常表达可能参与了结直肠癌的发生、发展,提示LINC01617可能作为判断结直肠癌进展的分子标志物及靶向治疗的分子靶点,其分子机制有待进一步研究。

Objective To investigate the clinical significance of long non-coding RNA LINC01617 in colorectal cancer tissues and corresponding paracancerous tissues and to investigate the effect of LINC01617 on proliferation and metastasis of colorectal cancer.Methods The expression of LINC01617 was detected with real-time PCR in 152 cases of colorectal carcinoma tissues and corresponding adjacent tissues collected from March 2007 to December 2011 in Oncology Department of Shuguang hospital.The relationship between LINC01617 expression level and the features and prognosis of survival in colorectal cancer patients were analyzed.The interference of LINC01617 by lentivirus was used to mimic the loss of function of LINC01617 in colorectal cancer cell lines.MTT was performed for cell proliferation and cell migration.Results LncRNA LINC01617 expression level in colorectal cancer was significantly higher than that in adjacent tissues(8.2±7.42 to 2.32±1.34,P<0.001),there was no correlation between LINC01617 expression and age,gender in colorectal cancer(P>0.05),while tumor size,lymph node metastasis and clinical pathological stage were significantly correlated(P<0.001,P<0.001,P=0.003).There was a significant negative correlation between the expression of LINC01617 and the survival rate of patients with colorectal cancer(P<0.01).Knockdown of LINC01617 expression could reduce the proliferation and metastasis of colorectal cancer cell line HCT116 and Lovo cells,and the differences were statistically significant(P<0.05).Conclusion The expression of lncRNA and LINC01617 in colorectal cancer tissues is abnormally high,and its abnormally high expression is related to the malignant clinical features and shorter prognosis survival of the patients.The effect that LINC01617 exert on colorectal cancer cell growth and promote metastasis showed that the abnormal expression of LINC01617 may be involved in the occurrence and development of colorectal cancer,suggesting that LINC01617 may act as a judge of the progression of colorectal cancer molecular markers and potential therapeutic target,its mechanisms should be further studied.