白细胞介素9在肾癌组织中的表达及其临床意义

Expression of interleukin-9 in renal tumor tissues and its clinical significance

目的探讨白细胞介素9(IL-9)在肾癌组织中的表达阳性率及临床意义。方法收集苏州大学附属第一医院66例行肾癌手术患者的组织标本,采用免疫组化法检测肾透明细胞癌组织和癌旁组织中IL-9的阳性表达情况并比较其差异,同时分析癌组织中IL-9的表达与患者临床病理特征之间的关系。结果在癌旁组织中,IL-9蛋白阳性表达61例、阴性表达5例;在肾透明细胞癌组织中,IL-9蛋白阳性表达7例、阴性表达59例,两者差异有统计学意义(P<0.01)。肾透明细胞癌组织中IL-9蛋白的表达水平与患者的R.E.N.A.L.评分(P=0.028)及分化程度(P=0.004)呈负相关,而与性别(P=0.072)、年龄(P=0.257)、体重质量指数(P=0.794)、肿瘤直径(P=0.419)及TNM分期(P=0.540)无关。结论肾癌组织中IL-9表达阳性率低于癌旁组织,且在肾癌组织中,R.E.N.A.L.评分越高或分化程度越差,则IL-9阳性表达越低,提示IL-9在肾癌组织微环境中可能具有一定的保护作用。

Objective To explore the positive expression level of IL-9 in renal tumor tissues and its clinical significance.Methods Renal tumor tissues as well as paired adjacent normal tissues were collected and then IL-9 expression level was detected by immunohistochemistry.Also,the correlation between IL-9 expression and the clinicopathological features was analyzed.Results The expression of IL-9 in renal tumor tissues was significantly lower than that in adjacent normal tissues(P<0.01).The correlation between IL-9 expression level and R.E.N.A.L.scores(P=0.028)as well as differentiation(P=0.004)was found negative.There was no significant difference in clinicopathological features including gender(P=0.072),age(P=0.257),BMI(P=0.794),tumor size(P=0.419)or TNM staging(P=0.540).Conclusions It was found that IL-9 positive expression in renal tumor tissues was lower than that in adjacent normal tissues.Low positive expression of IL-9 was detected when R.E.N.A.L.score was high or the cell differentiation was poor,suggesting that IL-9 may positively participate in the tumor microenvironment of renal tumor.