负载离子介孔二氧化硅纳米材料对牙本质小管封闭的耐磨性研究

Effect of ions-loaded mesoporous silica nanoparticles on dentinal tubules abrasion resistance

目的本研究通过体外实验,评价负载Ca^(2+)/PO_4^(3-)介孔二氧化硅纳米材料(Ca^(2+)/PO_4^(3-)@MSNs)对牙本质小管封闭的耐磨性。方法制备25片1mm厚的人牙本质片随机分为A组(空白对照)、B组(极固宁)、C组(MSNs)、D组(Ca^(2+)/PO_4^(3-)@MSNs体外混合)、E组(Ca^(2+)/PO_4^(3-)@MSNs体内混合)进行处理。每片再分为两半片,磨耗实验前后,一半扫描电镜观察横剖面计算牙本质小管堵塞率;另一半粘接剂处理,观察纵剖面,测量形成树脂突的长度。结果磨耗前B、C、D、E组的堵塞率没有统计学差异(P>0.05),A组树脂突长度与其余四组有差异,其余四组无树脂突。磨耗后B组堵塞率与C、D、E组有统计学差异(P <0.05),C、D、E组间堵塞率无统计学差异。树脂突长度A与B组间无统计学差异;A组与C、D、E组间有统计学差异;B组与C、D、E组间有统计学差异;C、D、E组各组间均无统计学差异(P>0.05)。结论 MSNs、Ca^(2+)/PO_4^(3-)@MSNs耐磨性能优于极固宁。Ca^(2+)/PO_4^(3-)@MSNs不同混合方法的即刻封闭效果及耐磨性无差异。

Objective To investigate the abrasion resistance of Ca2+/PO43-@MSNs on sealing dentinal tubules.Methods Twenty-five 1mm-thick human dentin slices were prepared and randomly divided into 5 groups,A:control group,B:green-OrTM,C:MSNs,D:in vitro mixture of Ca2+/PO43-@MSNs,E:in vivo mixture of Ca2+/PO43-@MSNs.Every slice was cut into two,and before and after dental abrasion test,dentinal occlusion was observed and caculated by SEM in one slice and another applied with adhesive and the length of resin tag measured.Results Before the abrasion test,plugging rate of group B,C,D and E showed no signifincant difference.There was signifincant difference between A and other four groups.After the abrasion test,plugging rate in group B was signifincantly different from those in group C,D,E.The length of resin tags in group A had no signifincant difference from group B.There were signifincant differences between group C,D,E and group A.Group B was statistical difference from group C,D,E.There was no statistical difference among group C,D,E.Conclusion MSNs and Ca2+/PO43-@MSNs have better abrasion resistance than that of Green-OrTM.There are no differences in occlusion effect and abrasion resistance between in vitro mixture and in vivo mixture of Ca2+/PO43-@MSNs.