基于核酸适体-纳米金银染放大的玉米赤霉烯酮快速检测方法研究

A Colorimetric Aptasensor for the Fast Detection of Zearalenone Based on AuNPs Combined with Argentation Amplification

玉米赤霉烯酮(Zearalenone,ZEA)具有较强的生殖毒性、致突变和致畸作用。以ZEA适体为识别元件,构建了基于纳米金诱导聚集和银染放大的ZEA适体比色可视化检测方法。结果表明,在优化条件下,ZEA浓度在5~200 ng/mL范围内与体系的吸光度值呈良好的线性关系,其线性回归方程为y=0.2486+0.00046156x(R2=0.9902),最低检测限为5 ng/mL,且方法特异性良好。进一步通过银染作用将该方法的灵敏度提高了50倍。经对比,该方法对实际样品的检测结果与酶联免疫法基本一致,为食品中ZEA的快速检测提供了简便有效的新策略。

Zearalenone(ZEA)has strong reproductive toxicity,mutagenicity and teratogenicity.A rapid visual aptasensor for sensitive and specific detection of zearalenone(ZEA)was developed in this study based on gold nanoparticle(AuNP)aggregation induced by the competitive absorption of aptamer between gold nanoparticle and zearalenone.The results showed that a good linear correlation between the concentration of ZEA and the absorbance of AuNPs was found within the range of 5-200 ng·mL-1,the linear regression equation is y=0.2486+0.00046156x(R2=0.9902).And the detection limit was 5 ng/ml with a good specificity.Furthermore,the sensitivity of the assay was further enhanced 50 times by combining with argentation amplification strategy.The reliability of the developed assay for detecting the actual corn and corn oil samples were verified by comparing with those detected by commercial ELISA kit.These results demonstrated that the developed assay has great potential in the rapid and specific detection of ZEA in foodstuffs.