日本血吸虫感染小鼠的脾脏CD8+PD-1+T细胞的功能特性

Functional characteristics of CD8+PD-1+T cell in spleen of mice infected with Schistosoma japonicum

目的研究日本血吸虫感染后小鼠脾脏CD8+PD-1+T细胞含量及功能的变化情况。方法使用日本血吸虫感染5~6周的C57BL/6小鼠,分离脾脏,制备石蜡切片,HE染色,观察组织病变情况;分离淋巴细胞并计数,运用流式细胞术检测CD8+PD-1+T细胞的含量;使用细胞表面染色的方法观察CD8+PD-1+T细胞的CD25、CD69、CXCR5、CD40L的表达变化;经PMA和离子霉素的刺激,使用细胞内细胞因子染色的方法检测IFN-γ、IL-10和IL-12的分泌情况。结果日本血吸虫感染后,小鼠体质量下降(P<0.05),而脾脏质量和体积均明显上升(P<0.01);脾脏中CD8+PD-1+T细胞的比例和绝对值数目增加(P<0.01);感染组CD8+PD-1+T细胞高表达CD69(P<0.05),而感染组CD8+PD-1+T细胞CXCR5分子表达显著下降(P<0.05);经PMA与离子霉素刺激以后,CD8+PD-1+T细胞IFN-γ分泌增加(P<0.05)。结论血吸虫感染5~6周小鼠脾脏中CD8+PD-1+T细胞为一群主要的活化细胞群可大量分泌IFN-γ,在血吸虫感染疾病发挥重要的免疫调节作用。

This study was performed to investigate the phenotypes and percentages change of CD8+PD-1+T cell in mouse spleen after Schistosoma japonicum(S.japonicum)infection.S.japonicum infetion model was constructed by using C57BL/6 mice.Representative images of spleen,images of and the spleen H&E staining were analyzed,while lymphocytes count were measured and the changes of CD8+PD-1+T cells were detected by flow cytometry.Data showed that the infectious mice exhibited cachexia symptoms,weight loss,and spleen weight and volume increase.Schistosoma japonicum infection also enhanced the accumulation of CD8+T cell and some phenotypic molecules,such as CD69,which were significantly higher than those in the control group.Furthermore,CD8+PD-1+Tsubset expressed more IFN-γafter Schistosoma japonicum infection.Taken together,this study reveals that CD8+PD-1+T cell in spleen is a group of major activated cell and secretes a large amount of IFN-γat 5-6 weeks after Schistosoma japonicum infection,and plays an important role in immune regulation in schistosomiasis infections.