响应面优化羟丙基-β-环糊精对血根碱的包合工艺

Optimization for Inclusion Technology of Sanguinarine HP-β-CD Inclusion Complex by Response Surface Methodology

本文采用溶液搅拌法制备血根碱与羟丙基-β-环糊精包合物。以羟丙基-β-环糊精和血根碱包合比、包合温度、包合时间为考察因素,在单因素试验基础上,应用Box-Behnken中心组合方法进行3因素3水平的试验设计,以血根碱和羟丙基-β-环糊精包合率为响应值,采用响应面法对包合条件进行进一步优化。结果表明:最佳条件为:包合比10∶1,包合温度60℃,包合时间190 min,血根碱和羟丙基-β-环糊精包合率为73.55%,与预测值(72.43%)接近(相对误差1.5%)。该方法稳定、重复性好,是一种高效制备血根碱羟丙基-β-环糊精包合物的方法。

In this study,solution-stirring method was used to prepare sanguinarine and HP-β-CD inclusion complex.Taking the inclusion ratio of HP-β-CD and sanguinarine,the inclusion temperature,and the inclusion time as the influencing factors,based on the single factor test,the Box-Behnken center combination method was used to perform 3 factors and 3 levels.The experimental design was based on the response values of sanguinarine and HP-β-CD inclusion rate,and the response surface method was used to further optimize the inclusion conditions.The results showed that the best conditions were:inclusion ratio 10∶1,inclusion temperature 60℃,inclusion time 190 min,The inclusion rate of sanguinarine and HP-β-CD was 73.55%,which was close to the predicted value(72.43%)(relative error 1.5%).The method was stable and repeatable,and was an efficient method for preparing sanguinarine HP-β-CD inclusion compound.