阿胶低聚肽的成分分析及其抗氧化活性

Composition and Its Antioxidant Activity of Colla Corii Asini Oligopeptides

采用复合酶解法制备阿胶低聚肽,分析阿胶低聚肽的营养成分、氨基酸组成和分子量分布,并比较阿胶与阿胶低聚肽清除自由基和防护H_2O_2诱导成纤维细胞氧化损伤的能力。结果表明,阿胶低聚肽含有8种人体必需氨基酸和2种半必需氨基酸,疏水性氨基酸占比较高,约占氨基酸总量的46.23%。阿胶低聚肽的相对分子质量主要集中在900 Da以下,约83.87%。阿胶和阿胶低聚肽对DPPH、ABTS自由基清除率的IC_(50)值分别为1.89和1.39、3.33和2.06 mg/mL。10 mg/mL阿胶和阿胶低聚肽的预处理可使H_2O_2诱导损伤的成纤维细胞存活率分别提高了6.72%和14.34%,SOD的活性分别提升了178.04%和497.88%,减少了细胞内ROS水平。表明,阿胶和阿胶低聚肽均可清除DPPH、ABTS自由基和减轻H_2O_2诱导的成纤维细胞的氧化损伤,且阿胶低聚肽的效果优于阿胶。

Colla Corii Asini(CCA)oligopeptides were prepared by complex enzymatic hydrolysis,and its nutritional components,amino acid composition and molecular weight distribution were analyzed,and the ability of CCA and CCA oligopeptides protecting fibroblasts from oxidative damage caused by H 2O 2 was compared.The results showed that CCA oligopeptides contained 8 essential amino acids and 2 semi-essential amino acids,and the hydrophobic amino acids accounted for a relatively high proportion,accounting for about 46.23%of the total amino acids.The relative molecular mass of CCA oligopeptides was mainly below 900 Da,about 83.87%.The IC 50 values of CCA and CCA oligopeptides for DPPH,ABTS were 1.89 and 1.39,3.33 and 2.06 mg/mL,respectively.Pretreatment with 10 mg/mL CCA and CCA oligopeptides could increase the survival rate of fibroblasts by 6.72%and 14.34%,and the activity of SOD by 178.04%and 497.88%,and intracellular ROS levels reduced.It showed that both CCA and CCA oligopeptides could scavenge DPPH,ABTS free radicals and reduce the oxidative damage of fibroblasts induced by H 2O 2,and the effect of CCA oligopeptides was better than CCA.