LPS联合烟熏致COPD大鼠模型的证候判定

Syndrome Judgment of COPD Rat Model Induced by LPS Combined with Smoking

目的从大鼠宏观表征定量化分析入手,以"因脉证治"为探索思路,分析LPS联合烟熏造成大鼠慢性阻塞性肺疾病的证候归属。方法将SD大鼠随机分为空白组和模型组。模型组采用LPS联合烟熏造模,通过病理、血气判断其COPD病理状态,记录造模完成后大鼠宏观表征变化并将数据定量化、四诊合参其证候归属。结果模型组大鼠平静及游泳后呼吸频率加快;体重减轻且增幅小;力竭游泳时间距离缩短、活动能力不足、四肢抓力下降;毛色亮度值偏低;舌色、爪色偏红、舌质暗红、舌下脉络迂曲的宏观表征符合气虚血瘀证主症、次症及舌脉的诊断标准。结论LPS联合烟熏可以稳定、重复、持续地造成大鼠气虚血瘀COPD证候模型。

Objective Starting from quantitative analysis of rat macroscopic characterization,and taking"cause-pulsesyndrome-treatment"as the exploratory thinking,to analyze the syndrome attribution of the COPD rat model which was caused by LPS combined with smoking.Methods SD rats were randomly divided into two groups(blank group and model group).The model group was established by LPS combined with smoking.After modeling,the pathological state of COPD was judged by pathology and blood gas analysis.The macroscopic characteristics of the rats were observed and quantified,and comprehensive analysis syndrome type by using four diagnostic methods.Results The rats in the model group had accelerated breathing rate in a calm state or in swimming,lost weight and increased by small degrees.The time and distance was shortened in exhaustive swimming,activity ability was insufficient,the brightness value of coat color was low.Livid claw,dark purple tongue,sublingual varices,which was the macroscopic characteristics that accorded with the diagnostic criteria of main symptom,minor symptom and tongue condition of Qi deficiency and blood stasis syndrome.Conclusion LPS combined with smoking can steadily,repeatly,and continuously establish COPD rat model of Qi deficiency and blood stasis syndrome.