基于网络药理学探讨黄芪-当归配伍活性成分改善血管内膜增生的作用机制

Mechanism of Astragali Radix-Angelicae Sinensis Radix compatibility active ingredients in improving intimal hyperplasia based on network pharmacology

目的采用网络药理学方法探讨黄芪-当归配伍改善血管内膜增生的药效物质和可能的作用靶点。方法利用TCMSP数据库,获取黄芪-当归的有效成分,利用Pharmmaper数据库搜集活性成分所对应的靶标。通过Genecards、DigSeE和OMIM数据库,收集血管内膜增生相关的靶标,并与药物作用靶标相比较,筛选出共同部分,作为药物成分作用的预测靶标。利用STRING获取预测靶蛋白之间的相互关系,根据相互关系大小筛选出核心靶标。利用Cytoscape 3.6.1软件,绘制"药物-成分-疾病-靶标"网络图、核心靶标相互作用网络图。用R语言进行核心靶标的KEGG通路富集分析和GO生物过程分析。结果收集到黄芪-当归药对的20个活性成分,共得到193个潜在药物作用靶点、487个潜在疾病靶点,主要作用于EGFR、ESR1、ALB、MAPK8、PGR等多个靶标,涉及PI3K-Akt、MAPK、Ras等多条信号通路以发挥抗血管内膜增生的作用。结论基于网络药理学方法,初步探讨了黄芪-当归配伍改善血管内膜增生可能的靶标和信号通路,可为黄芪-当归配伍作用机制的研究提供参考。

Objective To investigate the pharmacodynamics and possible targets of Astragali Radix(AR)-Angelicae Sinensis Radix(ASR)compatibility in improving intimal hyperplasia by using network pharmacology.Methods The TCMSP database was used to obtain the active constituents of AR-ASR,and the Pharmmaper database was used to collect the targets corresponding to the active ingredients.Targets related to intimal hyperplasia were collected by Genecards,DigSeE,and OMIM databases,and compared with drug targets,common parts were screened as predictive targets for drug action.The use of STRING was to obtain the predicted relationship between target proteins,and to screen out the core targets according to the size of the relationship.Using the Cytoscape 3.6.1 software,a"drug-component-disease-target"network map and a core target interaction network map were drawn.KEGG pathway enrichment analysis and GO biological process analysis of core targets were performed using R language.Results A total of 193 drug targets and 487 disease targets were obtained from the 20 active components of AR-ASR,mainly targeting EGFR,ESR1,ALB,MAPK8,PGR and other targets,involving PI3K-Akt,MAPK,Ras and other signaling pathways to play a role in anti-vascular intimal hyperplasia.Conclusion Based on the network pharmacology,the possible targets and signaling pathways for the improvement of intimal hyperplasia by AR-ASR can be preliminarily explored,which may provide a reference for the study of the mechanism of the combination of AR and ASR.