手性5-异丙基-2-噁唑烷酮的酶催化立体选择性互补合成

Enzymatic Stereocomplementary Synthesis of Chiral 5-Iisopropyloxazolidin-2-one

以2-异丙基环氧乙烷为底物,氰酸钠为亲核试剂,筛选获得两种卤醇脱卤酶HheA10和HheG,分别催化合成了(S)-5-异丙基-2-噁唑烷酮和(R)-5-异丙基-2-噁唑烷酮。结构经1H NMR和13C NMR表征,通过对其反应条件进一步考察,建立了立体选择性互补合成手性5-异丙基-2-噁唑烷酮的生物催化技术路线。

By using 2-isopropyloxirane as a substrate and cyanate as a nucleophilic reagent,we screened halohydrin dehalogenases and obtained two enzymes HheA10 and HheG,which catalyzed the formation of(S)-5-isopropyloxazolidin-2-one and(R)-5-isopropyloxazolidin-2-one respectively.The structures were characterized by 1H NMR and 13C NMR.By further investigation of the reaction conditions,we developed a biocatalytic route for stereocomplementary synthesis of chiral 5-isopropyloxazolidin-2-one.