细胞角蛋白17在不同HPV分型、宫颈病变组织表达及与HR-HPV感染关系

Expression of cytokeratin 17 in cervical lesions tissue of patients with different types of HPV cervical infection and its correlation with high-risk HPV infection

目的:探讨细胞角蛋白17(CK17)在不同人乳头瘤病毒(HPV)分型宫颈病变组织表达及与高危型HPV(HR HPV)感染关系。方法:收集2014年1月—2018年12月本院收治的200例液基细胞学异常组织标本,采用基因芯片导流杂交技术进行HPV分型检测,免疫组化法检测宫颈病变组织中CK17表达,比较不同病理分级宫颈病变、不同HPV分型宫颈病变组织中CK17表达差异,分析CK17表达与HR HPV关系。结果:不同病理分级宫颈病变者HPV阳性率存在差异(P<0.05);HPV共检出14个型,其中HR HPV 11个(16、18、31、33、45、51、52、53、58、66、68)、低危(LR)HPV3个(6、11、CP8304),不同宫颈病变中均HPV 16型均占比最高。慢性宫颈炎、CIN和宫颈癌组织中,CK17阳性表达率随病变级别增高而增强(P<0.05)。CK17阳性率HPV 16/18(51.6%)、52/58(33.3%)高于分型(25.8%),HR HPV感染者中CK17阳性率高于LR HPV感染者(均P<0.05);Spearman相关性分析,CK17表达与HR HPV呈正相关关系(r=0.176,P=0.022)。结论:CK17在不同HPV分型、宫颈病变组织中表达均存在差异,其表达水平与HR HPV感染呈弱正相关关系。

Objective:To investigate the expression of cytokeratin 17(CK17)in cervical lesions tissue of patients with different types of HPV cervical infection,and to study it’s relevant with high-risk HPV(HR-HPV)infection.Methods:200 samples with liquid-based cytology abnormalities of patients between January 2014 and December 2018 were collected.The type of HPV was detected by gene chip hybridization technology.CK17 expression in tissues of cervical lesions was detected by immunohistochemistry.The differences of CK17 expression were compared among patients with different pathological grades of cervical lesions and among patients with different cervical HPV type infection.The relationship between CK17 expression and HR HPV infection was analyzed.Results:There was a statistically significant difference in HPV positive rate among patients with different pathological grades of cervical lesions(P<0.05).A total of 14 HPV types were detected,which included 11 types of HR HPV(16,18,31,33,45,51,52,53,58,66,and 68)and 3 types of low risk HPV(6,11,and CP8304).The proportion of 16 type HPV infection of patients with different cervical lesions was the highest.In the tissues of chronic cervicitis,CIN or cervical cancer,the positive expression rate of CK17 was increased with the increasing of lesion grade(P<0.05).The CK17 positive expression rate in cervical tissues of patients with HPV 16/18 and 52/58 infection were 51.6%and 33.3%,which had statistically significant difference from that(25.8%)of other types of HPV infection.CK17 positive rate of patients with HR HPV infection was significant higher than that of patients with low risk HPV infection(all P<0.05).Spearman correlation analysis showed that CK17 expression was positively correlated with HR HPV infection(r=0.176,P=0.022).Conclusion:CK17 has expression difference in cervical lesions tissue of patients with cervical different types of HPV infection,and CK17 expression level has a weak positive correlation with HR HPV infection.