摘要
建立二维色谱-质谱联用技术(2D-LC-MS/MS)快速检测饮料中21种邻苯二甲酸酯类塑化剂的方法。饮料试样经乙腈-叔丁基甲醚(9︰1)提取后,直接进二维液相色谱-质谱联用仪分析。通过二维液相的第一维在线净化富集、第二维分离分析功能实现对21种邻苯二甲酸酯类塑化剂在线全自动的定性、定量分析。结果表明,21种邻苯二甲酸酯类塑化剂在8~1000 ng/mL范围内线性关系良好,相关系数R2均大于0.994,方法检出限范围为20~50μg/kg,在50~1000μg/kg加标水平下的回收率为85%~106%,相对标准偏差rSD(n=6)均小于10%。该方法操作简单、灵敏度高、分析时间短,适用于饮料中多种塑化剂的快速检测分析,可为国家相关标准制定提供参考依据。
Establish a two-dimensional liquid chromatography-mass spectrometry(2 D-LC-MS/MS)method for 21 phthalate esters in beverage.The beverage sample was extracted by acetonitrile-tert-butyl methyl ether(9︰1),then directly analyzed by two-dimensional liquid chromatography and mass spectrometry to realize the online automatic qualitative and quantitative analysis of 21 phthalate esters.Sample was purified and enriched through the first dimension of two-dimensional liquid phase,separated and analyzed through the second dimension.The results showed that the calibrations of 21 phthalate ester plasticizers were linear in the wide range of 8-1000 ng/mL with the correlation coefficients R2≥0.994.The limits of detection(LODs)were 20-50μg/kg.The average recoveries of the 21 phthalate esters in the range of 50-1000μg/kg were ranged from 85%to 106%.RSDs(Relative standard deviation)were less than 10%.This method was easy operated,highly sensitive and timeless,and could be used for the rapid detection and analysis of various plasticizers in beverages,to provide references for establishing relevant national standards.
作者
唐雷鸣
蒋玲波
王萍亚
陆羚子
戴意飞
黄鹂
TANG Leiming;JIANG Lingbo;WANG Pingya;LU Lingzi;DAI Yifei;HUANG Li(Zhoushan Institute for Food and Drug Inspection and Testing,Zhoushan 316021)
出处
《食品工业》
CAS
北大核心
2020年第8期304-307,共4页
The Food Industry
基金
浙江省科技厅分析测试计划项目(2018C37001)。