扬麦16籽粒灌浆速率相关性状的QTL定位(小麦15K SNP芯片法)

Detection of QTLs for Grain-Filling Rate Related Traits of Yangmai 16 Based on 15K SNP Array

为给选育籽粒灌浆快、粒重高的小麦新品种提供材料与技术支撑,以扬麦16、镇麦168、扬麦20和扬麦22为亲本构建获得的158个RIL群体为材料,利用小麦15K SNP芯片构建遗传连锁图谱,对小麦籽粒灌浆速率相关性状进行QTL定位。结果表明,应用完备区间作图法共检测到11个QTL,其中检测到5个与灌浆速率相关的新的QTL,分别位于3AL、4DL(2)、6AL和7AL上,可解释3.4%~9.3%的表型变异;检测到3个与千粒重相关的QTL,分别位于4AL(2)和4DS上,可解释3.9%~9.2%的表型变异;首次检测到3个与籽粒灌浆持续时间相关的QTL,分别位于3AL和4DL(2)上,可解释2.7%~7.5%的表型变异。扬麦16提供与灌浆速率和千粒重相关QTL的增效基因,累加了定位到的全部籽粒灌浆快和粒重高的位点;扬麦22提供与籽粒灌浆持续时间相关QTL的增效基因。扬麦16和扬麦22可用作选育早熟、大粒小麦新品种的亲本材料。

To study the genetic mechanism and dynamic QTLs for grain-filling rate related traits of Yangmai 16,the four-way RIL population constructed with Yangmai 16(YM16),Zhenmai 168(ZM168),Yangmai 20(YM20)and Yangmai 22(YM22)as parents was genotyped with wheat 15K SNP array to identify the QTL associated with grain-filling rate related traits.A total of 11 QTLs were detected by ICIM(inclusive composite interval mapping).5 new QTLs associated with grain-filling rate were detected on 3AL,4DL(2),6AL and 7AL chromosomes,explaining the phenotypic variation ranging from 3.4%to 9.3%;3 QTLs associated with grain weight were detected on 4AL(2)and 4DS chromosomes,explaining the phenotypic variation ranging from 3.9%to 9.2%;3 firstly reported QTLs associated with grain-filling period were detected on 3AL and 4DL(2),explaining the phenotypic variation ranging from 2.7%to 7.5%.YM16 contributed the favourable gene to all grain-filling rate and grain weight QTLs,and YM22 contributed the favourable gene to all grain-filling period QTLs.Moreover,YM16 was the cultivar responsible for all fast grain-filling and high grain weight QTLs detected.YM16 and YM22 can be used as parents materials for breeding new varieties of early maturing and large-grain wheat.