表没食子儿茶素没食子酸酯抑制ALL及Jurkat细胞株迁移侵袭的研究

Research on Epigallocatechin-3-Gallate(EGCG)inhibiting the migration of acute lymphoblastic leukemia cells

目的研究表没食子儿茶素没食子酸酯(EGCG)对Jurkat细胞和ALL患儿骨髓单个核细胞(BMMNC)迁移侵袭的抑制作用。方法以Jurkat细胞和ALL患儿BMMNC作为研究对象,给予不同浓度的EGCG处理,通过MTT法检测细胞增殖,流式细胞仪检测细胞凋亡率的变化,通过Transwell法检测Jurkat细胞和BMMNC的迁移和侵袭力。结果(1)经不同浓度EGCG处理不同时间后,Jurkat细胞、ALL患儿BMMNC的增殖均受到不同程度的抑制。各EGCG处理组的抑制率均明显高于对照组(P<0.05),且10、25、50、80、100μmol/L EGCG作用随着其浓度的增加和作用时间的延长,细胞抑制率逐渐增高,呈剂量和时间依赖关系。(2)经不同浓度EGCG处理不同时间后,不同程度地诱导了Jurkat细胞和BMMNC的凋亡。各EGCG处理组的凋亡率均明显高于对照组(P<0.05),且10、25、50、80、100μmol/L EGCG作用随着其浓度的增加和作用时间的延长,细胞凋亡率逐渐增高,呈剂量和时间依赖关系。(3)细胞迁移和侵袭实验中,Jurkat细胞和BMMNC经不同浓度EGCG预处理12、24、48h后,细胞的迁移穿膜细胞数和侵袭穿膜细胞数均明显减少,与对照组比较,有显著的差异(P<0.05),且呈现剂量和时间依赖性。结论EGCG能有效地抑制ALL肿瘤细胞的增殖、诱导其凋亡,并降低其迁移和侵袭能力。

Objective To study the inhibitory effect of EGCG on the migration of Jurkat cells and BMMNCs of ALL in children and its mechanism.Methods Jurkat cells and BMMNCs of ALL in children as the experimental cells were treated by different concentrations of EGCG.MTT assay was used to determine cell proliferation while flowcytometry was used to detect the apoptosis rates.Transwell assay was used to analyze the migration and invasion ability of Jurkat cells and BMMNCs.Results(1)Treated with different concentrations of EGCG for different time,Jurkat cells and BMMNCs of ALL in children were suppressed in proliferation to different degrees.The inhibition rate of each EGCG pretreatment group was significantly higher than that of the control group(P<0.05),and the effect of EGCG(10,25,50,80,100 mol/L)was increased with the increase of the concentration and time.The inhibition rate was dose and time dependent.(2)Treated with different concentrations of EGCG for different time,Jurkat cells and BMMNCs of ALL in children were induced to apoptosis to different degrees.The apoptosis rates of EGCG pretreatment groups were significantly higher than that of the control group(P<0.05),and the effect of EGCG(10,25,50,80,100 mol/L)was increased with the increase of concentration and time.The apoptosis rate was dose and time dependent.(3)In migration and invasion experiment,Jurkat cells and BMMNCs of ALL in children were treated with different concentrations of EGCG for 12,24 and 48h.The numbers of migration and invasion cells were significantly decreased,compared to the control group(P<0.05).The numbers were dose and time dependent.Conclusions EGCG revises the proliferation of ALL cells,induce apoptosis and inhibit malignant biological behaviors of ALL cells.