海拔对贯叶连翘挥发性化合物积累的影响

Effect of elevations on accumulation of volatile organic compounds in Hypericum perforatum

【目的】探明海拔对贯叶连翘挥发性化合物(volatile organic compounds,VOCs)积累的影响.【方法】以3个海拔(1000、2300和2800 m)生长的贯叶连翘的地上部分为材料,对乙酸乙酯和正丁醇萃取液进行GC-MS分离鉴定.【结果】3个海拔共分离鉴定127个VOCs,其中乙酸乙酯和正丁醇萃取液分别鉴定出100、59个,32个共同存在;乙酸乙酯萃取液中VOCs总相对含量随海拔升高而降低,而正丁醇萃取液中VOCs总相对含量在2300 m呈现最低;相对含量较高的VOCs包括1)butanoic acid,butyl ester(54.96%);2)γ-sitosterol(16.46%);3)propanoic acid,2-methyl-,butyl ester(16.37%);4)n-hexadecanoic acid(15.99%);5)tetratriacontane(13.83%)等.【结论】海拔对非极性和极性化合物种类和相对含量具有显著影响.对贯叶连翘在高海拔区域种植栽培、VOCs开发和利用提供一定的理论依据与技术参考.

【Objective】To investigate the effect of elevations on accumulation of volatile organic compounds(VOCs)in Hypericum perforatum.【Method】The ethyl acetate and n-butanol extracts from aerial parts at different elevations(1000,2300 and 2800 m)was separated and identified by GC-MS.【Result】Total 127 VOCs were separated from the three elevations,100 and 59 of them were respectively identified from ethyl acetate and n-butanol extracts,and 32 VOCs were co-existed.The relative content of VOCs in the ethyl acetate extracts showed a decrease with an elevation increase,while reached the lowest level in the n-butanol extracts at the 2300 m.The VOCs with higher relative content included:1)butanoic acid,butyl ester(54.96%);2)γ-sitosterol(16.46%);3)propanoic acid,2-methyl-,butyl ester(16.37%);4)n-hexadecanoic acid(15.99%);5)tetratriacontane(13.83%).【Conclusion】Elevations have a significant effect on types and relative content of polar-and nonpolar-VOCs.These findings will provide theoretical foundation and useful information for cultivation at high-elevation regions,exploitation and utilization of VOCs in H.perforatum.