干扰AKAP12调控G1/S期促进乳腺癌MCF-7细胞增殖

Interfering with AKAP12 promotes proliferation of breast cancer MCF-7 cells by regulating G1/S phase

目的研究干扰A型激酶锚定蛋白12(AKAP12)对人乳腺癌MCF-7细胞增殖能力的影响,并初步探讨其机制。方法采用携带干扰AKAP12核苷酸序列(shRNA-AKAP12)、空白对照(shRNA-NC)的慢病毒转染乳腺癌MCF-7细胞。Western blot检测转染后细胞AKAP12蛋白表达;CCK-8实验、克隆形成检测增殖能力;Western blot检测细胞周期相关蛋白激酶抑制蛋白(p21、p27)以及细胞周期进展蛋白CyclinD1表达。结果与shRNA-NC细胞相比较,shRNA-AKAP12细胞AKAP12蛋白表达量减少,CCK-8实验以及克隆形成结果显示,shRNA-AKAP12细胞增殖能力明显增强(P<0.000 1、P<0.001),与shRNA-NC细胞相比,shRNA-AKAP12细胞周期相关蛋白激酶抑制蛋白(p21、p27)表达下调,而细胞周期进展蛋白CyclinD1表达上调。结论研究表明干扰AKAP12基因可增加MCF-7细胞增殖能力,干扰AKAP12表达加快MCF-7细胞G1/S期转换,对于雌激素受体阳性乳腺癌临床治疗和预后评估有一定价值。

Objective To investigate the effect of interference with AKAP12 on the proliferation ability of human breast cancer MCF-7 cells and to explore its mechanism.Methods MCF-7 cells were exogenously transfected lentivirus with shRNA-AKAP12 and shRNA-NC, respectively. Western blot was conducted to examine the expression of AKAP12 protein in transfected cells. The proliferation ability of transfected cells were measured by CCK-8 assay and cell plate cloning assay. Western blot was used to detect the protein expression levels of cyclin-dependent kinase(CDK) inhibitors(p21, p27) and cell cycle progression-related CyclinD1.Results Compared to shRNA-NC cells, the expression of AKAP12 protein in shRNA-AKAP12 cells decreased. CCK-8 assay and colony formation assay showed that the cell proliferation ability of shRNA-AKAP12 cells increased significantly(P<0.000 1, P<0.001). Compared to the shRNA-NC cells, the expression of the cyclin-dependent kinase(CDK) inhibitors(p21, p27) were down-regulated in the shRNA-AKAP12 cells, while the cell cycle progression protein(CyclinD1) was up-regulated.Conclusion The study indicated that interference with AKAP12 gene could increase the proliferation ability of MCF-7 cells. It accelerated the G1-S phase transition of MCF-7 cells after interference with AKAP12 gene. Thus it is available to clinical treatment and prognosis evaluation of estrogen receptor-positive breast cancer.