基于多数据库分析FAM72A在肝癌中的表达及意义

Expression and clinical significance of FAM72A in liver cancer:an analysis based on multi-databases

目的基于多数据库探讨FAM72A在肝癌中的表达差异及临床意义。方法利用HPA数据库分析FAM72A在人正常组织表达概况和FAM72A在肿瘤细胞中的定位。采用GEPIA和Oncomine数据库分析FAM72A在人肝癌组织与人正常肝组织间的表达差异。利用LinkedOmics平台分析FAM72A表达量与肝癌患者临床病理特征的关系。Kaplan-Meier Plotter平台分析FAM72A表达与患者预后的关系。联合cBioPortal和STRING数据库构建FAM72A在肝癌中的共表达分子调控网络,并使用DAVID平台进行共表达基因的KEGG通路富集分析和GO生物途经富集分析。结果FAM72A在人体正常肝组织中低表达,在肿瘤细胞中主要定位于囊泡、细胞膜及细胞质中。FAM72A在肝癌组织中表达高于正常肝组织(P<0.05)。FAM72A表达量与患者年龄(P=0.0068)、病理分期(P=0.018)、T分期(P=0.022)、人种(P=0.034)相关。FAM72A高表达组患者与低表达组患者相比预后更差(P=0.00012)。FAM72A在肝癌中的共表达基因集主要参与DNA转录、DNA转录的调控、细胞分裂、细胞核分裂等过程(FDR<0.05)。KEGG通路富集分析显示这些基因参与了细胞周期、RNA转运、剪接体、DNA复制、P53信号通路、碱基切除修复等相关通路(FDR<0.05)。构建了肝癌中FAM72A高度相关共表达分子的调控网络。结论FAM72A在肝癌组织中高表达,且高表达FAM72A患者的预后较差。FAM72A表达量与人种、年龄、病理分期相关,有望成为肝癌靶向治疗的新靶点和判断肝癌预后的新标志物。

Objective To investigate the expression and clinical significance of family with sequence similarity 72 member A(FAM72 A)in hepatocellular carcinoma(HCC).Methods FAM72 A mRNA expression overview in normal tissues was performed by HPA database.GEPIA and Oncomine databases were used to analyze the different expression of FAM72 A in HCC and normal liver tissues.LinkedOmics platform was used to analyze the relationship between the expression level of FAM72 A and the pathological characteristics of HCC.Relationship between expression level of FAM72 A and prognosis was analyzed through Kaplan-Meier Plotter.c Bio Portal and STRING databases were used to establish the FAM72 A co-expression molecular network.KEGG pathway enrichment analysis and GO enrichment analysis were done using DAVID.Results FAM72 A was low expressed in normal liver tissues.FAM72 A expression in HCC tissues was higher than that in normal liver tissues(P<0.05).Expression of FAM72 A was correlated with age(P=0.0068),pathological stage(P=0.018),T stage(P=0.022)and race(P=0.034).Patients with high expression of FAM72 A tended to have poorer prognosis(P=0.00012).FAM72 A co-expression genes involved in biological process including DNA transcription,regulation of DNA transcription,cell division and nuclear division(FDR<0.05).KEGG pathway enrichment analysis showed that these genes were involved in cell cycle,RNA transport,spliceosome,DNA replication,P53 signaling pathway,base excision and repair,and other related pathways(FDR<0.05).FAM72 A co-expression molecular regulatory network was established.Conclusion Expression of FAM72 A in HCC is higher and the patients with high expression level of FAM72 A have poorer prognosis.Expression of FAM72 A is correlated with race,age and pathological stage.FAM72 A may be potential therapeutic target and potential prognostic biomarker of HCC.