白细胞介素-22在2型糖尿病患者外周血中的表达及意义

Expression and significance of interleukin-22 in peripheral blood of patients with type 2 diabetes mellitus

目的了解2型糖尿病(T2DM)患者外周血中白细胞介素-22(IL-22)、白细胞介素-22结合蛋白(IL-22BP)水平的变化及其临床意义。方法选取T2DM患者90例作为T2DM组研究对象,另选取40名正常健康人作为对照组。酶联免疫吸附法检测各组IL-22、IL-22BP含量,同时测定一般生化指标及糖尿病慢性并发症筛查。结果与正常对照组相比,T2DM组IL-22表达水平降低(P<0.001),而IL-22BP水平升高(P<0.01)。相关性分析显示,IL-22与IL-22BP、踝/臂血压比值(ABI)呈负相关(ρ=-0.273、-0.312,P<0.01),与腰臀比(WHR)、空腹血糖(FPG)、糖化血红蛋白(HbA1C)、胰岛素抵抗指数(HOMA-IR)呈正相关(ρ=0.209、0.282、0.243、0.241,P<0.05),IL-22BP与HbA1C、低密度脂蛋白胆固醇(LDL-C)呈不同程度负相关(ρ=-0.290、-0.208,P<0.05)。Logistic回归分析显示IL-22及IL-22BP水平是T2DM发病的相关因素。合并糖尿病肾脏病变组较无糖尿病肾脏病变组IL-22表达下降(P<0.01)而IL-22BP表达增加(P<0.05)。结论T2DM患者外周血清中IL-22表达降低而IL-22BP表达升高,IL-22及IL-22BP表达与血糖控制、T2DM及糖尿病肾脏病变发生具有一定的相关性。

Objective To investigate the changes and clinical significance of interleukin-22(IL-22)and interleukin-22 binding protein(IL-22 BP)in peripheral blood of patients with type 2 diabetes mellitus(T2 DM).Methods A total of 90 patients with T2 DM were selected as the research objects of type 2 diabetes group,and another 40 normal healthy people were selected as the control group.The levels of IL-22 and IL-22 BP were measured by enzyme-linked immunosorbent assay(ELISA),and biochemical indexes and chronic complications were recorded.Results Compared with the normal control group,the level of IL-22 expression in T2 DM group was lower(P<0.001),while the level of IL-22 BP was higher(P<0.01).Correlation analysis showed that IL-22 was negatively correlated with IL-22 BP and ankle brachial index(ABI)(ρ=-0.273,-0.312,P<0.01),positively correlated with waist hip ratio(WHR),fasting plasma glucose(FPG),glycosylated hemoglobin(HbA1 C)and homeostasic model assessment of insulin resistance(HOMA-IR)(ρ=0.209,0.282,0.243,0.241,P<0.05),IL-22 BP was negatively correlated with HbA1 C and low density lipoprotein cholesterol(LDL-C)to different degrees(ρ=-0.290,-0.208,P<0.05).Logistic regression analysis showed that IL-22 and IL-22 BP levels were related factors for the pathogenesis of T2 DM.Compared with non-diabetic nephropathy group,the expression of IL-22 in diabetic nephropathy group decreased(P<0.01),while the expression of IL-22 BP increased(P<0.05).Conclusion The level of IL-22 expression decreases and IL-22 BP expression increases in type 2 diabetic patients,which may be related to blood glucose control,T2 DM and diabetic nephropathy.