金花茶叶皂苷提取工艺优化及抑制脂肪酶活性研究

Extraction process optimization of saponin from the leaves of Camellia nitidissima Chi and its inhibition of lipase activity

以金花茶叶为研究对象,通过单因素试验和响应面法探讨了乙醇体积分数、料液比、提取温度与时间因素对皂苷提取效果的影响,并对其抑制胰脂肪酶的作用进行了研究。结果表明,金花茶叶皂苷的最佳提取工艺条件为:乙醇体积分数59%、料液比1∶39(g/mL)、提取温度60℃、提取时间124 min,该条件下皂苷得率为4.82%,与预测值接近;提取的皂苷在温度37℃、质量浓度6 mg/mL、pH 7.5、反应30 min下对胰脂肪酶抑制率可达84.76%。说明金花茶叶皂苷能有效抑制胰脂肪酶的活性,具有较好的降血脂作用。

By using single factor experiment and response surface methods,the effects of ethanol volume fraction,solid-liquid ratio,extraction temperature and time on extracting saponin from the leaves of Camellia nitidissima Chi were discussed,and the inhibitory effect of the extracts on pancreatic lipase was also studied.The optimal extraction conditons for saponin from C.nitidissima Chi were determined as follows:59%ethanol,1∶39(g/mL)as solid-liquid ratio,extracting at 60℃for 124 min.The yield of saponin was 4.82%under the optimized conditions,closing to the predicted result.The research results indicated that the saponin extracted with the mass concentration of 6 mg/mL(pH 7.5)at 37℃for 30 min,had the highest inhibiting effect on pancreatic lipase,and the inhibition rate was 84.76%.The results revealed that the saponin from leaves of C.nitidissima Chi could effectively inhibit the activity of lipase and might have excellent effect of lowing blood lipid.