提取溶剂对石榴籽油生物活性及纳米乳形成的影响

Effects of Extraction Solvents on the Biological Activities of Pomegranate Seed Oil and the Formation of Its Nanoemulsions

目的:探讨提取溶剂对石榴籽油(pomegranate seed oil,PSO)生物活性及纳米乳形成的影响。方法:选择5种有机溶剂,采用超声法提取PSO,以PSO的出油率、黄酮、多酚、石榴酸含量、抗氧化活性、纳米乳浊度及其抗菌活性为指标,采用主成分分析法筛选提取溶剂,并分析抗氧化活性、纳米乳浊度及抗菌活性与理化常数的相关性。结果:提取溶剂对PSO粘度、酸度、有效成分含量及生物活性有较大影响。PSO抗氧化活性与其皂化值及多酚含量呈显著正相关(P<0.05),纳米乳浊度与油的皂化值呈负相关(r=-0.798)。综合评分最高的提取溶剂为乙酸乙酯。结论:以PSO出油率、有效成分含量、生物活性及纳米乳成型性为指标综合评价提取溶剂,对PSO纳米新制剂研制有重要意义。

Objective:To investigate the effects of extraction solvents on the biological activities of pomegranate seed oil(PSO)and the formation of its nanoemulsions.Methods:Five organic solvents were employed to extract PSO by ultrasound method.The extraction solvent was selected using principle composition analysis,on the basis of the oil yield,the contents of flavonoid,polyphenol,and punicic acid,antioxidant activity of PSO,turbidity and antibacterial activity of the nanoemulsions.The correlation of biological activities and the turbidity of nanoemulsions with the physicochemical constants of PSO were analyzed,respectively.Results:The extraction solvents had a great influence on the viscosity,acidity,content of active ingredients and biological activities of PSO.The antioxidant activity of PSO had obviously positive correlation with its saponification value and polyphenol content(P<0.05).The turbidity of nanoemulsions was negatively related with the saponification value of oil(r=-0.798).Ethyl acetate gained the highest integrated score in principle component analysis.Conclusion:The extraction solvents were evaluated based on the oil yield,active components,biological activities,and the formation of nanoemulsions of PSO.This assessment system would be helpful to develop advanced nano-preparations of PSO.