响应面法优化超高压提取枸杞多糖工艺

Optimization of Extraction Technology of Lycium barbarum Polysaccharide by Ultrahigh Pressure Based on Response Surface Methodology

以枸杞为试验原料,以水为溶剂采用超高压提取枸杞多糖。在单因素实验基础上,通过响应面法研究了提取压力、保压时间、提取温度、液料比4个因素对枸杞多糖得率的影响,优化了提取工艺,并对枸杞多糖的体外抗氧化活性进行了研究。结果表明,超高压提取枸杞多糖的最佳工艺条件为:提取压力446 MPa、保压时间8 min、提取温度58℃、液料比23∶1 mL/mg,该条件下进行三次重复试验,枸杞多糖平均提取得率为9.52%,与预测值9.54%误差为0.21%。枸杞多糖对O2^-·、·OH有一定的清除能力,当枸杞多糖浓度为1 mg/mL时,对·OH的清除率达到76.98%±2.64%,对O2^-·的清除率达到73.02%±1.83%,且枸杞多糖浓度在0~1 mg/mL范围内与抗氧化活性呈正相关。本研究为枸杞多糖提供了一种新型的提取工艺。

Lycium barbarum polysaccharide was extracted by ultra-high pressure,and the response surface methodology(RSM)was used to optimize the process parameters.On the basis of single-factor experiments,pressure,time,temperature and liquid-material ratio were selected as four independent variables.An experimental design was carried out involving four variables at three levels.The antioxidant activity of polysaccharide from Lycium barbarum was further studied.The results showed that the optimum process for extraction of Lycium barbarum polysaccharide by ultrahigh pressure:The extraction pressure 446 MPa,extraction time 8 min,extraction temperature 58℃,liquid-material ratio 23∶1 mL/g,the estimated and observed values of maximal yield of polysaccharide was 9.54% and 9.52%,respectively.The relative error was 0.21%.The results showed that the antioxidant activity of polysaccharide showed a dose-dependent manner within the range of 0~1.0 mg/mL,and the scavenging activities of·OH and O2^-·reached 76.98%±2.64% and 73.02%±1.83%,respectively,when at concentration of 1.0 mg/mL.This study provided a new extraction technology for polysaccharides from Lycium barbarum.