基于肠道菌群探讨泡菜中乳酸菌防治非酒精性脂肪肝的研究

Prevention Effect of LAB from Kimchi on Non-alcoholic Fatty Liver Disease Based on Gut Microbiota

本文旨在探讨泡菜中益生菌对患有非酒精性脂肪肝病(Nonalcoholic fatty liver disease,NAFLD)的小鼠肠道菌群稳态和脂质水平的影响。采用蛋氨酸-胆碱缺乏饲料喂食C57BL/6J小鼠,构建NAFLD疾病小鼠模型。以市售泡菜为原料,对其中的益生菌进行分离并鉴定后,进一步增菌培养得到107 CFU/mL的菌液。将肝脂变模型小鼠分为自然恢复组和实验组,分别灌胃PBS和菌液,对照组灌胃PBS,每2周采集一次小鼠的粪便,检测菌群的多样性。结果表明:通过16S rDNA分子生物学特征鉴定从泡菜中分离得到的菌株为植物乳杆菌。肝脂变小鼠经灌胃菌液实验后,可显著提高(P<0.05)粪便中双歧杆菌和乳酸杆菌的含量,降低肠球菌、肠杆菌和脆弱拟杆菌的数量;HE染色发现实验组中脂肪粒径和肝脂含量较自然恢复组降低;血液检测表明实验组中谷草转氨酶、谷丙转氨酶和碱性磷酸酶的含量显著低于(P<0.05)自然恢复组;血清中甘油三酯和总胆固醇含量高于自然恢复组。结论:泡菜中分离的益生菌可显著改善NAFLD小鼠肠道菌群紊乱及异常肝脂水平状态。

The aim of this study was to investigate the effect of probiotics in kimchi on gut micrebiota and liver lipid levels in NAFLD mice.Methionine-choline deficiency diet was used to feed C57BL/6J mice,and a mouse model of NAFLD disease was successfully constructed and used as the research object.Commercial kimchi as the raw material,the probiotics were isolated and identified,and then further cultured to obtain 107 cfu/mL concentration of the bacteria solution.The model mice with hepatic liposis were divided into the natural recovery group and the experimental group,PBS and bacterial solution were given by gavage,the control group were given PBS.The feces of the mice were collected every 2 weeks to detect the diversity of the flora.The results showed that the strain isolated from kimchi was identified by the molecular biological characteristics of 16S rDNA as Lactobacillus plantarum.And the content of bifidobacteria and lactobacilli in feces could be significantly increased(P<0.05)while reduce the number of Enterococcus,Enterobacter and Bacteroides fragilis after intragastric bacteria in hepatic lipid mice.HE staining showed that the liver lipid content and fat particle size in the experimental group were lower than those in the natural recovery group.Blood test showed that the contents of glutamic-oxaloacetic transaminase,alanine aminotransferase and alkaline phosphatase in the experimental group was significantly lower(P<0.05)than the natural recovery group;serum triglycerides and total cholesterol levels were higher than the natural recovery group.Conclusion:The probiotics isolated from kimchi could significantly improve the intestinal flora disorder and abnormal liver lipid levels in NAFLD mice.