微小RNA的差异表达与胚胎质量的相关性

Correlation between differential expression of microRNA and quality of embryos

目的探讨微小RNA(micro RNA,miRNA)差异表达与胚胎质量的相关性,为胚胎的植入前选择提供新的依据。方法用TaqMan微小RNA阵列(MicroRNA Array)对8个囊胚样本进行miRNA表达谱检测,筛选稳定高表达且有价值的miRNA。扩大样本量后选择囊胚,针对筛选的miRNA进行逆转录PCR检测,同时采用二代测序平台检测胚胎的染色体异常,对结果进行分析比较。结果MicroRNA Array检测发现mir-720、mir-372、mir-886-3p和mir-512-3p表达量较高,而mir-145表达量较低,推测与早期胚胎发育相关。选择56个废弃囊胚,对上述5种miRNA进行检测,结果显示mir-145和mir-886-3p在染色体正常组中显著低表达。mir-145以相对表达量9作为阈值,mir-886-3p以相对表达量3作为阈值,其以上均未见胚胎染色体检测为正常者。结论特定的miRNA表达差异与胚胎染色体异常存在相关性,有可能作为一种新的胚胎选择的生物标记。

Objective To explore the correlation between microRNA(miRNA)differential expression and quality of embryo.Methods The miRNA expression profiles of 8 blastocysts were detected by a TaqMan microRNA array,and miRNAs with a stable expression were selected.Additional blastocysts were selected,and the candidate miRNA was detected by real-time PCR.Meanwhile,chromosomal abnormalities of the embryos were detected by using next-generation sequencing,and the results were compared.Results The expression of mir-720,mir-372,mir-886-3p and mir-512-3p was higher than that of miR-145,which suggested that mir-720,mir-372,mir-886-3p and mir-512-3p are related to early embryo development.The expression of miR-145 and mir-886-3p were significantly lower in the normal chromosome group.With the threshold values of above 9 and 3 for the relative expression of miR-145 and mir-886-3p,respectively,there was no embryo without a chromosomal abnormality.Conclusion There is a correlation between the expression level of specific miRNA and chromosomal abnormalities of embryos,which may be used as a novel biomarker for embryo selection.