健脾益气摄血颗粒对斑马鱼肠道出血模型的止血作用评价

Hemostatic Effect of Jianpi yiqi Shexue Granules on Zebrafish Intestinal Hemorrhage Mode

目的建立斑马鱼血小板计数减少并发肠道出血模型,观察健脾益气摄血颗粒对斑马鱼肠道出血的止血效果,为临床推广应用提供依据。方法在检测最大致死药物浓度后,用辛伐他汀诱导斑马鱼肠道出血模型,并以确定的健脾益气摄血颗粒56、167和500μg/ml 3个不同剂量为干预药物,以斑马鱼肠道出血发生率、肠道出血减少率以及止血率为评定指标,观察健脾益气摄血颗粒对斑马鱼肠道出血的止血效果。结果模型对照组斑马鱼肠道出血发生率为70%,经健脾益气摄血颗粒56、167和500μg/ml浓度干预后,斑马鱼肠道出血发生率分别为37%、40%和80%。其中,健脾益气摄血颗粒56μg/ml、167μg/ml两个剂量组与模型对照组比较,差异有统计学意义(P<0.05),表明健脾益气摄血颗粒中小剂量在浓度可明显减少斑马鱼肠道出血的发生率。模型对照组斑马鱼肠道内血小板计数为16.60个,经健脾益气摄血颗粒56、167和500μg/ml浓度干预后,斑马鱼肠道内血小板计数分别为7.60、8.00和15.50个,与模型对照组比较,差异有统计学意义(P分别为<0.01、0.01、0.05)。肠道止血作用分别为54%、52%和7%。表明健脾益气摄血颗粒中小剂量对斑马鱼肠道出血具有明显的止血效果。结论健脾益气摄血颗粒小剂量(56μg/ml)与中剂量(167μg/ml)不但能够明显减少斑马鱼肠道出血率,也有良好止血疗效。

Objective To establish a model of thrombocytopenia in zebrafish complicated with intestinal bleeding,and to observe the hemostatic effect of jianpi yiqi granules on intestinal bleeding in zebrafish,so as to provide a basis for clinical promotion and application.Methods After measuring the maximum lethal drug concentration,simvastatin was used to induce a zebrafish intestinal hemorrhage model,and the three different doses of jianpi yiqi granules 56,167 and 500μg/ml were determined as the intervention drugs.The hemostatic effect of jianpi yiqi granules on the intestinal hemorrhage of zebrafish was evaluated with the incidence of zebrafish intestinal hemorrhage,which also can reduce the intestinal bleeding and the hemostatic rate.Results The zebrafish intestinal hemorrhage rate in the model control group was 70%.After intervention with Jianpiyiqi blood-taking particles 56,167 and 500μg/ml,the zebrafish intestinal bleeding rates were 37%,40%and 80%,respectively.Among them,the two dose groups of 56μg/ml and 167μg/ml were statistically significant compared with the model control group(P<0.05).The results showed that the concentration of small dose of jianpi yiqi granules could significantly reduce the incidence of intestinal bleeding in zebrafish.The number of platelets in the intestinal tract of zebrafish in the model control group was 16.60,and the number of platelets in the intestinal tract of zebrafish was 7.60,8.00 and 15.50 respectively,after the intervention with concentrations of 56,167 and 500μg/ml,which was statistically significant compared with the model control group(P<0.01,0.01 and 0.05).Intestinal hemostasis was 54%,52%and 7%,respectively.The results showed that small dose of jianpi yiqi granules had obvious hemostatic effect on intestinal bleeding of zebrafish.Conclusion Small dose(56μg/ml)and medium dose(167μg/ml)of jianpi yiqi granules can not only significantly reduce the intestinal bleeding rate of zebrafish,but also have good hemostatic effect.