黄胸蓟马对不同花卉挥发物的行为反应初探

Preliminary Study on Behavioral Responses of Thrips hawaiiensis to Volatiles of Different Flowers

采用Y型嗅觉仪和四臂嗅觉仪法测定了黄胸蓟马对洋桔梗、杜鹃、百合及菊花4种花挥发物的行为反应,并进一步采用GS-MS方法分析了4种花的挥发物在成分及含量上的差异,以期探讨黄胸蓟马的寄主选择机制和危害机制。结果表明,黄胸蓟马雌成虫对不同花卉表现出稳定的偏好性,依次为洋桔梗>杜鹃>百合>菊花。通过GC-MS分析,在洋桔梗上共检测到38种化合物,其中2-丁烯醛含量最高,为47.06%;杜鹃上共检测到56种化合物,其中壬醛含量最高,为15.21%;百合上共检测到42种化合物,其中反-β-罗勒烯含量最高,为25.16%;菊花上共检测到44种化合物,其中菊油环酮含量最高,为30.50%。因此,黄胸蓟马对4种花卉不同的偏好性,可能与其挥发物成分的种类及含量差异密切相关。

In order to test the olfactory responses of T.hawaiiensis to different host plant volatiles and further explore the host selection and damage mechanism of this pest species,the behavioral responses of T.hawaiiensis to the volatiles of Eustoma grandiflorum,Rhododendron simsii,Lilium brownie and Dendranthema morifolium flowers were studied by Y-type and Four-arm olfactometers.In addition,the components and relative content of the volatiles from the four kinds of flowers were also analyzed by gas chromatography-mass spectrometry(GC-MS).The results showed that female adults of T.hawaiiensis performed steady preference to the flower volatiles(E.grandiflorum>R.simsii>L.brownie>D.morifolium).The volatile components from the four kinds of flowers were analyzed by GC-MS,and there were 38,56,42 and 44 components identified in the volatile profiles of E.grandiflorum,R.simsii,L.brownie and D.morifolium,respectively.2-butenal(47.06%),nonanal(15.21%),trans-β-ocimene(25.16%)and chrysanthenone(30.50%)were separately the most abundant components of the volatiles of E.grandiflorum,R.simsii,L.brownie and D.morifolium.Thus,the different olfactory responses of T.hawaiiensis to the four flowers may be closely correlated with the differences of flower volatiles types and concentrations.