快速霉菌酵母测试片法与GB 4789.15—2016在3种类型食品中霉菌计数比较

Comparison of the rapid yeast and mold count plate method with GB 4789.15-2016 method for enumeration of molds in three types of food

目的比较快速霉菌酵母测试片(RYM)法和GB 4789.15—2016《食品安全国家标准食品微生物学检验霉菌和酵母计数》方法(以下简称国标法)测定3种类型食品样品中霉菌计数的一致性。方法选取90份糕点、面包、饼干样品,30份含乳饮料、植物蛋白饮料、固体饮料样品,30份保健食品样品并对3种类型食品样品进行人工污染得到的人工污染样品,利用RYM法和国标法进行霉菌计数的测定,检测结果通过配对资料t检验以及对数值差值绝对值(|dlog|)汇总分析进行统计。结果两种方法对3种类型食品样品以及人工污染样品检测结果在统计学意义上无显著性差异(P>0.05);|dlog|≤0.50占比均为100.0%,表明两种方法的检测结果一致性较好。结论RYM法和国标法对3种类型的食品样品及其人工污染样品中霉菌计数的检测结果一致性较好。

Objective The rapid yeast and mold count plate(RYM)method and the national standard(GB 4789.15-2016)method were compared for the consistency in three types of food detection.Methods 90 pastry,bread and biscuits samples,30 milk beverage,vegetable protein beverage and solid drink samples,30 health foods samples,three types of artificially contaminated food samples were detected by the RYM method and the GB method,then the data of the two method were analyzed by the paired t test and the log difference(|dlog|).Results The statistics were made for the data of three types of food samples and three types of artificially contaminated food samples.The paired t test gave P>0.05,and the ratio of|dlog|≤0.50 were 100.0%.There was no significant difference between the result of the two method.Conclusion There is no significant difference between the results of the RYM method and the GB method in detecting three types of food samples and three types of artificially contaminated food samples.