细胞模型在临床营养研究中应用现状

Application status of cell models in clinical nutrition research

传统临床营养研究多停留在人群和动物试验阶段,随着临床营养研究的深入发展,细胞模型试验也逐渐成为临床营养研究中的重要方式。本文主要综述了临床营养中的细胞模型试验常用的细胞系,详细阐述了细胞模型在临床营养中的应用现状,涉及动脉粥样硬化性心脑血管疾病、肌肉衰减综合征、肾脏疾病、肿瘤、胃肠疾病、肝损伤、过敏性炎症、烧伤等领域,就我国居民死因排名靠前的4种疾病做了重点阐述,分别指出各种疾病临床营养研究中常用细胞模型、细胞模型试验的应用优势及其科学意义,以及细胞模型发展应用趋势(三维细胞、细胞组、微流控、高通量高内涵),总结了临床营养中传统细胞模型试验的局限性,并对细胞模型试验中新技术新方法的应用前景进行展望。

Traditional clinical nutrition research mostly stay at the stage of human and animal experiments.Cell model experiment has gradually become an important way in clinical nutrition research nowadays.Firstly,this review mainly summarizes the cells commonly used in nutrition research and gives a general introduction of diseases in clinical nutrition using cell models such as atherosclerotic cardio-cerebrovascular disease,sarcopenia,kidney diseases,tumor,gastrointestinal diseases,liver damage,allergic inflammation,burns and so on.Then it focuses mainly on the diseases which are the leading death causes of the Chinese residents,reviewing the use of cell models the advantages as well as their scientific significance and the development and application trend of cell model(3 D cell,cytome,microfluidic,high throughput and high content).Finally,the limitations of cell model experiments in clinical nutrition are summarized,and new technologies used in cell model experiments of clinical nutrition are prospected.