摘要
本文建立了食品中酸性橙Ⅱ号的测试方法。仪器使用Agilent 1260infinity型号的液相色谱仪系统,氨基阴离子交换固相萃取柱,C18柱(250 mm×4.6 mm,填料粒径5μm)色谱柱,流动相为A相甲醇,B相为0.02 mol·L^-1乙酸铵水溶液,流速1.0 mL·min^-1,检测波长484 nm。结果表明,方法检出限和加标回收率均符合规定要求。该方法快速、准确、回收率高,对食品中的酸性橙Ⅱ号检测适用性高。
A method for the determination of Acid OrangeⅡin food was established.the method uses Agilent 1260 infinity liquid chromatograph system,NH2 Anionic exchange solid phase extraction column purification,the column was C18(250 mm×4.6 mm,5μm)with the mobile phase consisting of methanol and 0.02 mol·L^-1 ammonium acetate-water,the carrier gas flow rate was 1.0 mL·min^-1,the detection wavelength was set at 484 nm.The results showed that the detection limit and recovery of the method met the requirements.The method is rapid,accurate,with high recovery rate,and has high applicability for the detection of Acid OrangeⅡin food.
作者
张梦茹
Zhang Mengru(Intertek Testing Services Co.,Ltd.,Shanghai 200233,China)
出处
《现代食品》
2020年第16期222-224,共3页
Modern Food
