摘要
(R)-1,3-butanediol is an important pharmaceutical intermediate, and the synthesis of(R)-1,3-butanediol using green biological methods has recently been of interest for industrial application. Here, a novel strain QC-1 that efficiently transforms 4-hydroxy-2-butanone to(R)-1,3-butanediol was isolated from soil samples. Based on morphological, physiological, and biochemical tests and 5.8 S-internal transcribed spacer sequencing, the strain was identified as Pichia kudriavzevii QC-1. The reaction conditions were optimized to 35 ℃, pH 8.0, rotation speed 200 rpm, and 6:5 mass ratio of glucose to 4-hydroxy-2-butanone. Evaluation of the effects of 4-hydroxy-2-butanone concentrations on yield and cell survival rate showed that 85.60 g·L^-1 product accumulated, with an enantiomeric excess of more than 99%, when 30 g·L^-14-hydroxy-2-butanone was added at 0, 10, and 30 h in a 3-L bioreactor. Thus, strain QC-1 showed excellent catalytic activity and stereoselectivity for the synthesis of(R)-1,3-butanediol from 4-hydroxy-2-butanone.
基金
Financial supports from the National Key R&D Program of China(2018YFC1604100)
the National Natural Science Foundation of China(NSFC)[21676120,31872891]
the 111 Project[111-2-06]
the Highend Foreign Experts Recruitment Program[G20190010083]
the Program for Advanced Talents within Six Industries of Jiangsu Province[2015-NY007]
the National Program for Support of Top-notch Young Professionals
the Fundamental Research Funds for the Central Universities[JUSRP51504]
the Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions
Top-notch Academic Programs Project of Jiangsu Higher Education Institutions
the Jiangsu Province“Collaborative Innovation Center for Advanced Industrial Fermentation”Industry Development Program
the National First-Class Discipline Program of Light Industry Technology and Engineering[LITE2018-09]。