口腔颌面肿瘤活检标本对化疗药物敏感性的研究

Chemosensitivity testing of oral and maxillofacial cancer with biopsy specimens

目的 检测口腔颌面部癌肿活检标本的体外化疗药物敏感性 ,明确临床常用各种化疗药物对口腔癌敏感性差别。方法 1 50例口腔癌化疗前活检新鲜组织标本行改良MTT药敏检测。检测用药是顺铂、5 氟尿嘧啶、平阳霉素、紫杉醇、替尼泊苷、表阿霉素、长春地辛和甲氨蝶呤 8种常用化疗药物。结果 1 50例标本成功完成MTT药敏检测的有 1 4 0例 ,检测成功率是 93 33 %。MTT药敏检测所用药物浓度是各药物峰血浆浓度的 5倍。结果表明 ,8种药物对口腔癌细胞体外平均生长抑制率分别是 :替尼泊苷 63 76 %、顺铂 2 5 93 %、表阿霉素 2 5 86 %、紫杉醇 2 3 52 %、平阳霉素 2 2 97%、氟尿嘧啶 2 2 0 8%、长春地辛 1 8 42 %、甲氨蝶呤 1 8 93 % ;替尼泊苷对口腔颌面恶性肿瘤体外抗瘤效果显著高于其他 7种化疗药物 (P <0 0 5)。 >40 %的口腔鳞状细胞癌标本对替尼泊苷、顺铂、表阿霉素 3种药物中度以上敏感 ;>40 %的腺源性口腔癌标本对替尼泊苷、紫杉醇和表阿霉素中度以上敏感。结论 大多数口腔颌面部癌肿对替尼泊苷敏感 ,顺铂、表阿霉素和紫杉醇敏感性也较高。替尼泊苷、表阿霉素和紫杉醇是口腔颌面部恶性肿瘤有潜力的化疗药物 ,其敏感性强于长春地辛、氟尿嘧啶和甲氨蝶呤。临床上应用改良MTT药敏检测法 ,筛选口腔颌面?

Objective To determine the chemosensitivity in fresh biopsy specimen of human oral and maxillofacial cancer, and the differential chemosensitivity among those drugs used popularly in clinic. Methods Human biopsy cancer cells were obtained from 150 oral and maxillofacial malignant tumors. The antitumor drugs tested using modified MTT assay were cisplatin (CDDP), 5 fluorouracil(5 Fu), Pinyangmycin (PYM), Paclitaxel(Taxol), Teniposide(Vm 26), Epi adriamycin(E ADM), Vindesin (VDS) and Methortrexatum(MTX). Results The success rate of the MTT assay was 93 33% (140 of the 150 cases). At a drug concentration of Cmax×5, the inhibition rates of oral tumor cells were 63 76% for Vm 26, 25 93% for CDDP, 25 86% for E ADM, 23 52% for Taxol, 22 97% for PYM, 22 08% for 5 Fu, 18 42% for VDS and 18 93% for MTX The inhibition rate of VM26 was significantly higher than any of other seven chemotherapeutic drugs( P < 0 05). Over forty percent patients with squamous cell carcinoma showed moderate chemosensitivity to VM 26, CDDP and E ADM, and over forty percent cases with adenoid carcinoma showed moderate chemosensitivity to Vm 26, Taxol and E ADM. Conclusions Most oral and maxillofacial cancers showed chemosensitivity to Vm 26, CDDP, E ADM and Taxol. Vm 26, E ADM and Taxol were more potent drugs than VDS, 5 Fu and MTX against oral and maxillofacial cancer cells. Chemosensitivity testing using modified MTT assay was useful in selecting antitumor drugs for patients with oral and maxillofacial cancers.