山东地区人群中EB病毒感染情况的血清流行病学分析

Epidemiological study of Epstein-Barr virus infection in Shandong province

目的了解山东地区人群中EB病毒感染的血清流行病学情况。方法选取2015年9月至2018年8月在山东大学附属省立医院就诊的EB病毒疑似感染者9 445例。用化学发光免疫分析方法检测患者血清中抗EB病毒衣壳抗原抗体免疫球蛋白(Ig) G(VCA Ig G)、抗EB病毒衣壳抗原抗体Ig M(VCA Ig M)、抗EB病毒早期抗原抗体Ig G(EA-D Ig G)和抗EB病毒核抗原抗体Ig G(EBNA Ig G)情况。结果 9 445例患者中VCA Ig G阳性7 821例(82.81%),VCA Ig M阳性935例(9.90%),EA-D Ig G阳性513例(5.43%),EBNA Ig G阳性7 311例(77.41%)。男性患者VCA Ig G、EA-D Ig G、EBNA Ig G阳性率均低于女性,差异均有统计学意义(均P <0.05)。不同年龄患者间VCA Ig G、VCA Ig M、EA-D Ig G、EBNA Ig G阳性率差异均有统计学意义(均P <0.001)。VCA Ig M阳性率冬季最高为12.58%(279/2 217),秋季为11.45%(247/2 158),夏季为8.56%(230/2 686),春季最低为7.51%(179/2 384),季节之间差异有统计学意义(P <0.05)。共检出16种EB病毒抗体组合模式,其中以VCA Ig G、EBNA Ig G双阳性模式最多见,共检出6 235例,占66.01%。结论山东地区人群EB病毒抗体阳性率存在性别、年龄和季节差异,EB病毒抗体组合感染模式以VCA Ig G和EBNA Ig G双阳性的既往感染模式为主,其他多种感染模式并存。

Objective To investigate the prevalence of Epstein-Barr( EB) virus infection in the population of Shandong. Methods From September 2015 to August 2018,9 445 suspected EB virus patients admitted to Shandong Provincial Hospital Affiliated to Shandong University were collected. Serum EB virus capsid antigen antibody( VCA) immunoglobulin( Ig) G( VCA Ig G),VCA-IgM,EB virus early antigen-D antibody Ig G( EA-D Ig G) and EB virus nuclear antigen antibody Ig G( EBNA Ig G) were detected by chemiluminescence immunoassay.Results In 9 445 cases,the positive rates of VCA Ig G,VCA Ig M,EA-D Ig G and EBNA Ig G were 82. 81%( 7 821/9 445),9. 90%( 935/9 445),5. 43%( 513/9 445) and 77. 41%( 7 311/9 445),respectively. The positive rates of VCA Ig G,EA-D Ig G and EBNA Ig G in male patients were significantly lower than those in the female patients( all P < 0. 05). There were significant differences in the positive rates of VCA Ig G,VCA Ig M,EA-D Ig G and EBNA Ig G among patients of different ages( all P < 0. 001). The positive rate of VCA Ig M was12. 58%( 279/2 217) in winter,11. 45%( 247/2 158) in autumn and 8. 56%( 230/2 686) in summer,but7. 51%( 179/2 384) in spring( P < 0. 05). A total of 16 combinations of EB virus antibody combinations were detected,VCA Ig G and EBNA Ig G double positive pattern was the most common,a total of 6 235 cases( 66. 01%)were detected. Conclusions The positive rate of EB virus antibodies in Shandong shows differences in gender,age and season. The predominant infection patterns of VCA Ig G and EBNA Ig G double positive are complicated.