PSMA6基因rs7157054位点多态性与脑梗死的相关性研究

Correlation study between rs7157054 polymorphism of PSMA6 gene and cerebral infarction

目的探讨陕西汉族人群中PSMA6基因rs7157054位点的多态性与脑梗死的相关性。方法采用病例-对照研究,选择156例脑梗死患者(病例组)和150例健康体检者(对照组)为对象,应用聚合酶链反应-限制性片段长度多态性分析方法(PCR-RFLP)检测PSMA6基因rs7157054位点单核苷酸多态性,并分析其基因型及等位基因频率在脑梗死患者及正常人群中的分布特点。结果病例组G G基因型频率为50%(78/156),对照组频率为56%(84/150),两组之间差异没有统计学意义(Х^2=l.105,P=0.293)。A等位基因频率在病例组为27.9%(87/312),在对照组为24.0%(72/300),两组之间差异没有统计学意义(Х^2=1.200,P=0.273)。结论本研究提示PSMA6基因rs7157054位点GG基因型与A等位基因频率与脑梗死之间没有相关性关系。

Objective To discuss the correlation between rs7157054 polymorphism of PSMA6 gene and cerebral infarction in Han population of the Shaanxi province in china.Methods Adopting case-control method,the study selected 156 cerebral infarction patients(the case group)and 150 healthy controls(the control group).The single nucleotide polymorphism of rs7157054 of PSMA6 gene was identified by the polymerase chain reaction(PCR)and restriction fragment length polymorphism(RFLP),and genotype and allele frequency distributions in cerebral infarction patients and normal people were analyzed.Results The frequencies of GG genotype was 50%(78/156)in the case group and 56%(84/150)in the control group respectively.Chi-square test showed the trend of significant difference between the two groups(=1.105,P=0.293).The frequencies of A allele was 327.9%(87/312)in the case group,and 24.0%(72/300)in the control group respectively,which showed significant differences between the two groups(Х^2=1.200,P=0.273).Conclusion There was no correlation between GG genotype and A allele and cerebral infarction.