γδT细胞在慢性布鲁氏菌病患者外周血中的数量变化分析

Analysis of quantitative changes ofγδT cells in the peripheral blood of patients with chronic brucellosis

目的:探讨γδT细胞在慢性布鲁氏菌病患者抗菌治疗前后外周血中的数量变化。方法:于2019年9月,选择2012年9月至2018年4月就诊于天津市第二人民医院的88例慢性布鲁氏菌病患者为研究对象,选择2014年该院职工体检健康的30人作为健康对照组。对抗布鲁氏菌治疗前、治疗后6周、12周,外周血中γδT及CD3+、CD4+、CD8+T淋巴细胞计数和CD4/8比值数量变化进行分析。结果:与治疗前比较,患者完成6周抗布鲁氏菌病治疗后CD3+、CD4+和CD8+T淋巴细胞计数均明显降低,差异有统计学意义(P<0.05);患者完成12周抗布鲁氏菌治疗后γδT细胞、CD3+和CD8+T淋巴细胞计数均明显降低、CD4/8明显升高,差异均有统计学意义(P<0.05)。与健康对照组比较,患者治疗前γδT细胞、CD8+和CD3+T淋巴细胞计数较高、CD4/8计数较低,差异均有统计学意义(P<0.05);患者完成6周抗布鲁氏菌病治疗后,γδT细胞计数较高、CD4+T淋巴细胞计数较低,差异均有统计学意义(P<0.05);患者完成12周抗布鲁氏菌病治疗后γδT细胞计数较高,差异有统计学意义(P<0.01)。结论:γδT细胞、CD4+、CD8+和CD3+T淋巴细胞可能在机体抵制布鲁氏菌慢性感染中发挥作用。

Objective To investigate the quantitative changes ofγδT cells in peripheral blood before and after anti-Brucella treatment in patients with chronic brucellosis.Methods A prospective design was used to 88 patients with chronic brucellosis who were admitted to the Second People's Hospital of Tianjin from September 2012 to April 2018.The patients took anti-brucella drugs,And the changes in the number ofγδT cell,CD3+,CD4+,CD8+T lymphocytes and CD4/8 in peripheral blood before treatment,6 weeks and 12 weeks after treatment were analyzed.Thirty volunteers were selected as the healthy control group from Tianjin Second People's Hospital employee health checkup in 2014.Results After 6 weeks antibacterial therapy,the counts of CD3+,CD4+and CD8+T lymphocytes were significantly lower than before treatment in patients with chronic brucellosis(P<0.05).After 12 weeks antibacterial therapy,the counts ofγδT cell,CD3+,CD4+and CD8+T lymphocytes were significantly lower than before treatment(P<0.05),but CD4/8 was higher than before treatment in patients with chronic brucellosis(P<0.05).Compared with healthy control group,after 6 weeks antibacterial treatment,theγδT cell count was still significantly higher,but the CD4+T lymphocyte count was lower(P<0.05).After 12 weeks treatment,theγδT cell count was still significantly higher than that of the healthy control group(P<0.01).ConclusionγδT cells,CD4+,CD8+and CD3+T lymphocytes may play a role in human body resistance to chronic Brucella infection.