大鼠宫腔粘连模型的构建

The establishment of intrauterine adhensions model in rats

目的探讨建立大鼠宫腔粘连模型的方法,为进一步研究宫腔粘连的发生机制及探索治疗方法提供实验基础。方法60只SD雌性大鼠随机分为四组,每组15只。其中,A组作为对照组,大鼠宫腔注射PBS 0.6 mL,停留30 s后回抽关腹;B、C、D组大鼠宫腔注射无水乙醇0.6 mL,分别停留30 s、1 min和3 min后回抽关腹。造模术后第1个及第3个动情期后四组各处死6只大鼠取材,行HE染色,观察子宫内膜厚度及腺体数目,行Masson染色观察子宫内膜纤维化程度。取SD雄性大鼠6只,剩余雌鼠随机与雄鼠按2∶1合笼,妊娠第10天开腹计数子宫内胚胎数目。结果B、C、D组造模术后第1个动情期后子宫手术侧出现不同程度的损伤,子宫内膜变薄,腺体减少,且第3个动情期后无明显改善;C、D组上述改变更严重,子宫内膜完全破坏,无可修复潜能。与A组相比,B、C、D组造模术后第1个动情期后手术侧子宫内膜腺体数目均减少,子宫内膜纤维化面积比均增加(P<0.05),第3个动情期后无显著改善。B组手术侧子宫内胚胎数目少于A组(P<0.05),而C、D组手术侧子宫内均未见胚胎。结论动情期SD大鼠予无水乙醇宫腔停留30 s可成功构建大鼠宫腔粘连模型。该动物模型能反映宫腔粘连的组织学及病理学变化,适用于宫腔粘连的相关研究。

Objective To establish intrauterine adhesions model in SD rats for providing the experimental basis in studying the mechanism of intrauterine adhesion and exploring the therapeutic methods.Methods Sixty female SD rats were randomly divided into four groups with 15 rats each.The rats of group A were intrauterinely injected with PBS 0.6 mL,which was kept for 30 seconds and withdrawn thenafter.The rats in groups of B,C,D were intrauterinely injected with anhydrous ethanol 0.6 mL,which was kept in the uterus for 30 seconds,1 minute and 3 minutes and withdrawn thenafter,respectively.After the first and third estrus period,6 rats from each group were killed and the uterus was taken out for observing the endometrial thickness and number of glands using HE staining and the degree of endometrial fibrosis using Masson staining.The remaining female rats were randomly caged with male SD rats in a ratio of 2∶1,and the number of embryos in the uterus was counted by laparotomy on the 10th day of pregnancy.Results In groups of B,C and D,different degrees of damage occurred on the uterine side,the endometrium became thinner,and glands decreased after the first estrus period,and there was no significant improvement after the third estrus period.The above changes were more serious,the endometrium was completely destroyed,and there was no repair potential.Compared with group A,the endometrial glands numbers of the surgical side were decreased and endometrial fibrosis area ratio was increased after the first estrus period in groups of B,C,and D(P<0.05),and no significant improvement occured in the third estrus period.The number of uterine embryos in group B was less than that in group A(P<0.05),but no embryos were found in groups of C and D.Conclusion The model with intrauterine adhesion in SD rats can be successfully established by keeping anhydrous ethanol in the uterine cavity for 30 seconds in estrous period,which can reflect the histological and pathological changes of intrauterine adhesion and is suitable for the study of intrauterine adhesion.